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Functional N-methyl-D-aspartate receptors (NMDARs) are heteromultimers formed by NR1 and NR2 subunits. The M3 segment, as contributed by NR1, forms the core of the extracellular vestibule, including binding sites for channel blockers, and represents a critical molecular link between ligand binding and channel opening. Taking advantage of the substituted cysteine accessibility method along with channel block and multivalent coordination, we studied the contribution of the M3 segment in NR2C to the extracellular vestibule. We find that the M3 segment in NR2C, like that in NR1, contributes to the core of the extracellular vestibule. However, the M3 segments from the two subunits are staggered relative to each other in the vertical axis of the channel. Compared to NR1, homologous positions in NR2C, including those in the highly conserved SYTANLAAF motif, are located about four amino acids more externally. The staggering of subunits may represent a key structural feature underlying the distinct functional properties of NMDARs.
Amar,
High-affinity Zn block in recombinant N-methyl-D-aspartate receptors with cysteine substitutions at the Q/R/N site.
2001, Pubmed,
Xenbase
Amar,
High-affinity Zn block in recombinant N-methyl-D-aspartate receptors with cysteine substitutions at the Q/R/N site.
2001,
Pubmed
,
Xenbase Anson,
Identification of amino acid residues of the NR2A subunit that control glutamate potency in recombinant NR1/NR2A NMDA receptors.
1998,
Pubmed Antonov,
Binding sites for permeant ions in the channel of NMDA receptors and their effects on channel block.
1998,
Pubmed Ayalon,
Functional assembly of AMPA and kainate receptors is mediated by several discrete protein-protein interactions.
2001,
Pubmed
,
Xenbase Beck,
NMDAR channel segments forming the extracellular vestibule inferred from the accessibility of substituted cysteines.
1999,
Pubmed
,
Xenbase Benveniste,
Trapping of glutamate and glycine during open channel block of rat hippocampal neuron NMDA receptors by 9-aminoacridine.
1995,
Pubmed Burnashev,
Control by asparagine residues of calcium permeability and magnesium blockade in the NMDA receptor.
1992,
Pubmed
,
Xenbase Chatterton,
Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits.
2002,
Pubmed
,
Xenbase Chiamvimonvat,
Depth asymmetries of the pore-lining segments of the Na+ channel revealed by cysteine mutagenesis.
1996,
Pubmed
,
Xenbase Dingledine,
The glutamate receptor ion channels.
1999,
Pubmed Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed Hirai,
The glycine binding site of the N-methyl-D-aspartate receptor subunit NR1: identification of novel determinants of co-agonist potentiation in the extracellular M3-M4 loop region.
1996,
Pubmed Hollmann,
Cloned glutamate receptors.
1994,
Pubmed Holm,
Structural and Functional Aspects of Metal Sites in Biology.
1996,
Pubmed Horenstein,
Protein mobility and GABA-induced conformational changes in GABA(A) receptor pore-lining M2 segment.
2001,
Pubmed
,
Xenbase Jones,
The NMDA receptor M3 segment is a conserved transduction element coupling ligand binding to channel opening.
2002,
Pubmed
,
Xenbase Karlin,
Emerging structure of the nicotinic acetylcholine receptors.
2002,
Pubmed Karlin,
Substituted-cysteine accessibility method.
1998,
Pubmed Kashiwagi,
Channel blockers acting at N-methyl-D-aspartate receptors: differential effects of mutations in the vestibule and ion channel pore.
2002,
Pubmed
,
Xenbase Kohda,
Mutation of a glutamate receptor motif reveals its role in gating and delta2 receptor channel properties.
2000,
Pubmed Krupp,
N-terminal domains in the NR2 subunit control desensitization of NMDA receptors.
1998,
Pubmed Kuner,
Structure of the NMDA receptor channel M2 segment inferred from the accessibility of substituted cysteines.
1996,
Pubmed
,
Xenbase Kuryatov,
Mutational analysis of the glycine-binding site of the NMDA receptor: structural similarity with bacterial amino acid-binding proteins.
1994,
Pubmed
,
Xenbase Laube,
Molecular determinants of agonist discrimination by NMDA receptor subunits: analysis of the glutamate binding site on the NR2B subunit.
1997,
Pubmed Leszkiewicz,
Enhancement of NMDA receptor-mediated currents by light in rat neurones in vitro.
2000,
Pubmed Liu,
Dynamic rearrangement of the outer mouth of a K+ channel during gating.
1996,
Pubmed Liu,
Gated access to the pore of a voltage-dependent K+ channel.
1997,
Pubmed Loring,
Aromatic trivalent arsenicals: covalent yet reversible reagents for the agonist binding site of nicotinic receptors.
1992,
Pubmed Mansour,
Heteromeric AMPA receptors assemble with a preferred subunit stoichiometry and spatial arrangement.
2001,
Pubmed Paoletti,
Mechanosensitivity of NMDA receptors in cultured mouse central neurons.
1994,
Pubmed Robert,
Subunit interactions and AMPA receptor desensitization.
2001,
Pubmed Rosenmund,
The tetrameric structure of a glutamate receptor channel.
1998,
Pubmed Sobolevsky,
Two-component blocking kinetics of open NMDA channels by organic cations.
1999,
Pubmed Sobolevsky,
Two blocking sites of amino-adamantane derivatives in open N-methyl-D-aspartate channels.
1998,
Pubmed Sobolevsky,
Molecular rearrangements of the extracellular vestibule in NMDAR channels during gating.
2002,
Pubmed
,
Xenbase Sobolevsky,
Probing of NMDA channels with fast blockers.
1999,
Pubmed Solomon,
Multicopper Oxidases and Oxygenases.
1996,
Pubmed Sprengel,
Glutamate receptor channel signatures.
2001,
Pubmed Subramaniam,
Hydrophobic interactions of n-alkyl diamines with the N-methyl-D-aspartate receptor: voltage-dependent and -independent blocking sites.
1994,
Pubmed Villarroel,
Glycine-independent NMDA receptor desensitization: localization of structural determinants.
1998,
Pubmed Vissel,
Intracellular domains of NR2 alter calcium-dependent inactivation of N-methyl-D-aspartate receptors.
2002,
Pubmed Vlachová,
Copper modulation of NMDA responses in mouse and rat cultured hippocampal neurons.
1996,
Pubmed Wollmuth,
Differential contribution of the NR1- and NR2A-subunits to the selectivity filter of recombinant NMDA receptor channels.
1996,
Pubmed
,
Xenbase Wollmuth,
Intracellular Mg2+ interacts with structural determinants of the narrow constriction contributed by the NR1-subunit in the NMDA receptor channel.
1998,
Pubmed
,
Xenbase Yang,
Molecular determinants of Ca2+ selectivity and ion permeation in L-type Ca2+ channels.
1993,
Pubmed
,
Xenbase
