Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Xenopus have been powerful model organisms for understanding vertebrate development and disease for over 100 years. Here, a rapid blood perfusion protocol in Xenopus, aimed at a consistent and drastic reduction of blood within all tissues, is defined. Perfusion is carried out by inserting a needle directly into the ventricle of the heart and pumping heparinized phosphate-buffered saline (PBS) through the vascular system. The procedure can be completed in approximately 10 min per animal. The blood is dominated by a few highly abundant proteins and cell types, creating numerous issues as these proteins mask most other molecules and cell types of interest. The reproducible characterization of adult Xenopus tissues with quantitative proteomics and single-cell transcriptomics will benefit from applying this protocol prior to organ sampling. The protocols for tissue sampling are defined in companion papers. These procedures are aimed at the standardization of practices across Xenopus of different sex, age, and health status, specifically X. laevis and X. tropicalis.
???displayArticle.pubmedLink???
37318240 ???displayArticle.pmcLink???PMC11233139 ???displayArticle.link???J Vis Exp ???displayArticle.grants???[+]
Briggs,
The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution.
2018, Pubmed,
Xenbase
Briggs,
The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution.
2018,
Pubmed
,
Xenbase Heinz-Taheny,
Cardiovascular physiology and diseases of amphibians.
2009,
Pubmed Hoops,
A perfusion protocol for lizards, including a method for brain removal.
2015,
Pubmed Lametschwandtner,
Microvascular anatomy of the urinary bladder in the adult African clawed toad, Xenopus laevis: A scanning electron microscope study of vascular casts.
2021,
Pubmed
,
Xenbase Lametschwandtner,
Microvascular anatomy of the brain of the adult pipid frog, Xenopus laevis (Daudin): A scanning electron microscopic study of vascular corrosion casts.
2018,
Pubmed
,
Xenbase Lametschwandtner,
Microvascular anatomy of the non-lobulated liver of adult Xenopus laevis: A scanning electron microscopic study of vascular casts.
2022,
Pubmed
,
Xenbase Liao,
Cell landscape of larval and adult Xenopus laevis at single-cell resolution.
2022,
Pubmed
,
Xenbase Miodoński,
Arterial supply of the choriocapillaris of anuran amphibians (Rana temporaria, Rana esculenta). Scanning electron-microscopic (SEM) study of microcorrosion casts.
1987,
Pubmed Navarro,
Bupivacaine as a euthanasia agent for African Clawed Frogs (Xenopus laevis).
2022,
Pubmed
,
Xenbase Nenni,
Xenbase: Facilitating the Use of Xenopus to Model Human Disease.
2019,
Pubmed
,
Xenbase Saltman,
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum.
2017,
Pubmed Stephenson,
The vertebrate heart: an evolutionary perspective.
2017,
Pubmed Tandon,
Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling.
2017,
Pubmed
,
Xenbase Wu,
Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation.
2021,
Pubmed
