Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2002 Nov 15;2122:6216-24. doi: 10.1093/emboj/cdf620.
Show Gene links
Show Anatomy links
Exportin-5-mediated nuclear export of eukaryotic elongation factor 1A and tRNA.
Calado A, Treichel N, Müller EC, Otto A, Kutay U.
???displayArticle.abstract???
Transport of proteins and RNA into and out of the cell nucleus is mediated largely by a family of RanGTP-binding transport receptors. Export receptors (exportins) need to bind RanGTP for efficient loading of their export cargo. We have identified eukaryotic elongation factor 1A (eEF1A) and tRNA as RanGTP-dependent binding partners of exportin-5 (Exp5). Exp5 stimulates nuclear export of eEF1A when microinjected into the nucleus of Xenopus laevis oocytes. Surprisingly, the interaction between eEF1A and Exp5 is dependent on tRNA that can interact directly with Exp5 and, if aminoacylated, recruits eEF1A into the export complex. These data suggested to us that Exp5 might support tRNA export. Indeed, not only the canonical tRNA export receptor, exportin-t, but also Exp5 can drive nuclear export of tRNA. Taken together, we show that there exists an alternative tRNA export pathway which can be exploited to keep eEF1A out of the cell nucleus.
Arts,
Identification of a nuclear export receptor for tRNA.
1998, Pubmed,
Xenbase
Arts,
Identification of a nuclear export receptor for tRNA.
1998,
Pubmed
,
Xenbase Arts,
The role of exportin-t in selective nuclear export of mature tRNAs.
1998,
Pubmed
,
Xenbase Azad,
Role of nuclear pools of aminoacyl-tRNA synthetases in tRNA nuclear export.
2001,
Pubmed Billaut-Mulot,
Trypanosoma cruzi elongation factor 1-alpha: nuclear localization in parasites undergoing apoptosis.
1996,
Pubmed Blondel,
Nuclear export of Far1p in response to pheromones requires the export receptor Msn5p/Ste21p.
1999,
Pubmed Brownawell,
Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins.
2002,
Pubmed Cullen,
Retroviruses as model systems for the study of nuclear RNA export pathways.
1998,
Pubmed DeVit,
The nuclear exportin Msn5 is required for nuclear export of the Mig1 glucose repressor of Saccharomyces cerevisiae.
1999,
Pubmed Ejiri,
Moonlighting functions of polypeptide elongation factor 1: from actin bundling to zinc finger protein R1-associated nuclear localization.
2002,
Pubmed Feng,
A Los1p-independent pathway for nuclear export of intronless tRNAs in Saccharomycescerevisiae.
2002,
Pubmed Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase Fukuda,
CRM1 is responsible for intracellular transport mediated by the nuclear export signal.
1997,
Pubmed
,
Xenbase Gadal,
Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p.
2001,
Pubmed Gangwani,
Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells.
1998,
Pubmed Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed Grosshans,
Review: transport of tRNA out of the nucleus-direct channeling to the ribosome?
2000,
Pubmed Grosshans,
An aminoacylation-dependent nuclear tRNA export pathway in yeast.
2000,
Pubmed Grüter,
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
1998,
Pubmed
,
Xenbase Hellmuth,
Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA.
1998,
Pubmed Ho,
Nmd3p is a Crm1p-dependent adapter protein for nuclear export of the large ribosomal subunit.
2000,
Pubmed Hurt,
Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing.
1987,
Pubmed Iborra,
Coupled transcription and translation within nuclei of mammalian cells.
2001,
Pubmed Izaurralde,
The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus.
1997,
Pubmed Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase Kaffman,
The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus.
1998,
Pubmed Kjaer,
Generation and epitope mapping of high-affinity scFv to eukaryotic elongation factor 1A by dual application of phage display.
2001,
Pubmed Kuersten,
Nucleocytoplasmic transport: Ran, beta and beyond.
2001,
Pubmed Kutay,
Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor.
1997,
Pubmed
,
Xenbase Kutay,
Identification of a tRNA-specific nuclear export receptor.
1998,
Pubmed
,
Xenbase Kutay,
Identification of two novel RanGTP-binding proteins belonging to the importin beta superfamily.
2000,
Pubmed Lipowsky,
Coordination of tRNA nuclear export with processing of tRNA.
1999,
Pubmed
,
Xenbase Lund,
Proofreading and aminoacylation of tRNAs before export from the nucleus.
1998,
Pubmed
,
Xenbase Macara,
Transport into and out of the nucleus.
2001,
Pubmed Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed Mühlhäusser,
Multiple pathways contribute to nuclear import of core histones.
2001,
Pubmed Negrutskii,
Eukaryotic translation elongation factor 1 alpha: structure, expression, functions, and possible role in aminoacyl-tRNA channeling.
1998,
Pubmed Ohno,
PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation.
2000,
Pubmed
,
Xenbase Roobol,
The role of guanine nucleotides in the interaction between aminoacyl-tRNA and elongation factor 1 of Artemia salina.
1978,
Pubmed Sanders,
Immunofluorescence studies of human fibroblasts demonstrate the presence of the complex of elongation factor-1 beta gamma delta in the endoplasmic reticulum.
1996,
Pubmed Sarkar,
Nuclear tRNA aminoacylation and its role in nuclear export of endogenous tRNAs in Saccharomyces cerevisiae.
1999,
Pubmed
,
Xenbase Sarkar,
tRNA nuclear export in saccharomyces cerevisiae: in situ hybridization analysis.
1998,
Pubmed
