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Methylation of the ribose 2'-hydroxyl, the most widespread modification of ribosomal and splicesomal RNAs, is guided by the box C/D class of small nucleolar RNAs (snoRNAs). Box C/D small nucleolar ribonucleoproteins (snoRNPs) contain four core proteins: fibrillarin, Nop56, Nop58 and 15.5 kDa. We constructed U25 snoRNAs containing a single photoactivatable 4-thiouridine at each U position within the conserved box C/D and C'/D' motifs. Proteins assembled on the snoRNA after injection into Xenopus oocyte nuclei were identified by cross-linking, and reconstituted particles characterized by functional rescue and mutational analyses. Our data argue that box C/D snoRNPs are asymmetric, with the C' box contacting Nop56 and fibrillarin, the C box interacting with Nop58, and the D and D' boxes contacting fibrillarin. No cross-link to 15.5 kDa was detected; its binding is disrupted by 4-thiouridine substitution in position 1 of the C box. Repositioning the guide sequence of U25 upstream of box D instead of D' revealed that both C/D motifs have the potential to function as guide centers, but, surprisingly, there was no alteration in protein cross-linking.
Anderson,
Processing of adenovirus 2-induced proteins.
1973, Pubmed
Anderson,
Processing of adenovirus 2-induced proteins.
1973,
Pubmed Balakin,
The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions.
1996,
Pubmed Bortolin,
Elements essential for accumulation and function of small nucleolar RNAs directing site-specific pseudouridylation of ribosomal RNAs.
1999,
Pubmed Caffarelli,
In vivo identification of nuclear factors interacting with the conserved elements of box C/D small nucleolar RNAs.
1998,
Pubmed
,
Xenbase Cavaillé,
Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides.
1996,
Pubmed Filipowicz,
Biogenesis of small nucleolar ribonucleoproteins.
2002,
Pubmed Filippini,
p62, a novel Xenopus laevis component of box C/D snoRNPs.
2000,
Pubmed
,
Xenbase Ganot,
Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs.
1997,
Pubmed Gaspin,
Archaeal homologs of eukaryotic methylation guide small nucleolar RNAs: lessons from the Pyrococcus genomes.
2000,
Pubmed Gautier,
Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis.
1997,
Pubmed Hüttenhofer,
RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse.
2001,
Pubmed Kiss,
Small nucleolar RNA-guided post-transcriptional modification of cellular RNAs.
2001,
Pubmed Kiss-László,
Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA.
1998,
Pubmed Kiss-László,
Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.
1996,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Lafontaine,
Synthesis and assembly of the box C+D small nucleolar RNPs.
2000,
Pubmed Lafontaine,
Nop58p is a common component of the box C+D snoRNPs that is required for snoRNA stability.
1999,
Pubmed Lapeyre,
Molecular cloning of Xenopus fibrillarin, a conserved U3 small nuclear ribonucleoprotein recognized by antisera from humans with autoimmune disease.
1990,
Pubmed
,
Xenbase Lund,
Nonaqueous isolation of transcriptionally active nuclei from Xenopus oocytes.
1990,
Pubmed
,
Xenbase Lyman,
Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins.
1999,
Pubmed Maden,
The numerous modified nucleotides in eukaryotic ribosomal RNA.
1990,
Pubmed Moore,
Site-specific modification of pre-mRNA: the 2'-hydroxyl groups at the splice sites.
1992,
Pubmed Newman,
Box C/D snoRNA-associated proteins: two pairs of evolutionarily ancient proteins and possible links to replication and transcription.
2000,
Pubmed Ni,
Small nucleolar RNAs direct site-specific synthesis of pseudouridine in ribosomal RNA.
1997,
Pubmed Nottrott,
Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5' stem-loop of U4 snRNA.
1999,
Pubmed Omer,
In vitro reconstitution and activity of a C/D box methylation guide ribonucleoprotein complex.
2002,
Pubmed Omer,
Homologs of small nucleolar RNAs in Archaea.
2000,
Pubmed Peculis,
Disruption of U8 nucleolar snRNA inhibits 5.8S and 28S rRNA processing in the Xenopus oocyte.
1993,
Pubmed
,
Xenbase Prives,
Use of oligonucleotides for antisense experiments in Xenopus laevis oocytes.
1991,
Pubmed
,
Xenbase Reimer,
Monoclonal autoantibody from a (New Zealand black x New Zealand white)F1 mouse and some human scleroderma sera target an Mr 34,000 nucleolar protein of the U3 RNP particle.
1987,
Pubmed Romero,
Expression cloning using Xenopus laevis oocytes.
1998,
Pubmed
,
Xenbase Schimmang,
A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.
1989,
Pubmed Sontheimer,
Site-specific RNA crosslinking with 4-thiouridine.
1994,
Pubmed Speckmann,
Archaeal guide RNAs function in rRNA modification in the eukaryotic nucleus.
2002,
Pubmed
,
Xenbase Terns,
Small nucleolar RNAs: versatile trans-acting molecules of ancient evolutionary origin.
2002,
Pubmed Terns,
3'-end-dependent formation of U6 small nuclear ribonucleoprotein particles in Xenopus laevis oocyte nuclei.
1992,
Pubmed
,
Xenbase Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed Tycowski,
A small nucleolar RNA requirement for site-specific ribose methylation of rRNA in Xenopus.
1996,
Pubmed
,
Xenbase Tycowski,
Modification of U6 spliceosomal RNA is guided by other small RNAs.
1998,
Pubmed
,
Xenbase Venema,
Ribosome synthesis in Saccharomyces cerevisiae.
1999,
Pubmed Vidovic,
Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment.
2000,
Pubmed Watkins,
A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP.
2000,
Pubmed Watkins,
In vitro assembly of the mouse U14 snoRNP core complex and identification of a 65-kDa box C/D-binding protein.
1998,
Pubmed
,
Xenbase Watkins,
Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure.
1998,
Pubmed Weinstein,
Guided tours: from precursor snoRNA to functional snoRNP.
1999,
Pubmed Wu,
Nop5p is a small nucleolar ribonucleoprotein component required for pre-18 S rRNA processing in yeast.
1998,
Pubmed Yu,
A new method for detecting sites of 2'-O-methylation in RNA molecules.
1997,
Pubmed
,
Xenbase
