Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Translational activation of several dormant mRNAs in vertebrate oocytes is mediated by cytoplasmic polyadenylation, a process controlled by the cytoplasmic polyadenylation element (CPE) and its binding protein CPEB. The translation of CPE-containing mRNAs does not occur en masse at any one time, but instead is temporally regulated. We show here that in Xenopus, partial destruction of CPEB controls the temporal translation of CPE-containing mRNAs. While some mRNAs, such as the one encoding Mos, are polyadenylated at prophase I, the polyadenylation of cyclin B1 mRNA requires the partial destruction of CPEB that occurs at metaphase I. CPEB destruction is mediated by a PEST box and Cdc2-catalyzed phosphorylation, and is essential for meiotic progression to metaphase II. CPEB destruction is also necessary for mitosis in the early embryo. These data indicate that a change in the CPEB:CPE ratio is necessary to activate mRNAs at metaphase I and drive the cells' entry into metaphase II.
Andrésson,
The kinase Eg2 is a component of the Xenopus oocyte progesterone-activated signaling pathway.
1998, Pubmed,
Xenbase
Andrésson,
The kinase Eg2 is a component of the Xenopus oocyte progesterone-activated signaling pathway.
1998,
Pubmed
,
Xenbase Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997,
Pubmed
,
Xenbase Bally-Cuif,
Characterization of the zebrafish Orb/CPEB-related RNA binding protein and localization of maternal components in the zebrafish oocyte.
1998,
Pubmed
,
Xenbase Barkoff,
Meiotic maturation in Xenopus requires polyadenylation of multiple mRNAs.
1998,
Pubmed
,
Xenbase Barkoff,
Translational control of cyclin B1 mRNA during meiotic maturation: coordinated repression and cytoplasmic polyadenylation.
2000,
Pubmed
,
Xenbase de Moor,
The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes.
1997,
Pubmed
,
Xenbase de Moor,
Cytoplasmic polyadenylation elements mediate masking and unmasking of cyclin B1 mRNA.
1999,
Pubmed
,
Xenbase Dickson,
The cleavage and polyadenylation specificity factor in Xenopus laevis oocytes is a cytoplasmic factor involved in regulated polyadenylation.
1999,
Pubmed
,
Xenbase Fenwick,
A subclass of Ras proteins that regulate the degradation of IkappaB.
2000,
Pubmed Ferby,
A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes.
1999,
Pubmed
,
Xenbase Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase Groisman,
CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: implications for local translational control of cell division.
2000,
Pubmed
,
Xenbase Gross,
The critical role of the MAP kinase pathway in meiosis II in Xenopus oocytes is mediated by p90(Rsk).
2000,
Pubmed
,
Xenbase Hake,
Specificity of RNA binding by CPEB: requirement for RNA recognition motifs and a novel zinc finger.
1998,
Pubmed
,
Xenbase Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Hauser,
A giant ubiquitin-conjugating enzyme related to IAP apoptosis inhibitors.
1998,
Pubmed Hochegger,
New B-type cyclin synthesis is required between meiosis I and II during Xenopus oocyte maturation.
2001,
Pubmed
,
Xenbase Katsu,
Ca2+ is required for phosphorylation of clam p82/CPEB in vitro: implications for dual and independent roles of MAP and Cdc2 kinases.
1999,
Pubmed King,
How proteolysis drives the cell cycle.
1996,
Pubmed Laney,
Substrate targeting in the ubiquitin system.
1999,
Pubmed Lenormand,
Speedy: a novel cell cycle regulator of the G2/M transition.
1999,
Pubmed
,
Xenbase McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase Mendez,
Phosphorylation of CPEB by Eg2 mediates the recruitment of CPSF into an active cytoplasmic polyadenylation complex.
2000,
Pubmed
,
Xenbase Mendez,
Translational control by CPEB: a means to the end.
2001,
Pubmed
,
Xenbase Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase Minshall,
Dual roles of p82, the clam CPEB homolog, in cytoplasmic polyadenylation and translational masking.
1999,
Pubmed
,
Xenbase Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase Murray,
Cell cycle extracts.
1991,
Pubmed Nakahata,
Biochemical identification of Xenopus Pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a Nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein.
2001,
Pubmed
,
Xenbase Nebreda,
Regulation of the meiotic cell cycle in oocytes.
2000,
Pubmed
,
Xenbase Peter,
The APC is dispensable for first meiotic anaphase in Xenopus oocytes.
2001,
Pubmed
,
Xenbase Rechsteiner,
PEST sequences and regulation by proteolysis.
1996,
Pubmed Reverte,
CPEB degradation during Xenopus oocyte maturation requires a PEST domain and the 26S proteasome.
2001,
Pubmed
,
Xenbase Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase Salic,
Control of beta-catenin stability: reconstitution of the cytoplasmic steps of the wnt pathway in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase Stebbins-Boaz,
Maskin is a CPEB-associated factor that transiently interacts with elF-4E.
1999,
Pubmed
,
Xenbase Stutz,
Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes.
1998,
Pubmed Swenson,
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Taieb,
Activation of the anaphase-promoting complex and degradation of cyclin B is not required for progression from Meiosis I to II in Xenopus oocytes.
2001,
Pubmed
,
Xenbase Tay,
The control of cyclin B1 mRNA translation during mouse oocyte maturation.
2000,
Pubmed van der Geer,
Phosphopeptide mapping and phosphoamino acid analysis by electrophoresis and chromatography on thin-layer cellulose plates.
1994,
Pubmed Walker,
The clam 3' UTR masking element-binding protein p82 is a member of the CPEB family.
1999,
Pubmed
,
Xenbase
