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The analysis of protein interactions with chromatin is vital for the understanding of DNA sequence recognition in vivo. Chromatin binding requires the interaction of proteins with DNA lying on the macromolecular protein surface of nucleosomes, a situation that can alter factor binding characteristics substantially when compared with naked DNA. It is therefore important to study these protein-DNA interactions in the context of a chromatin substrate, the more physiologically relevant binding situation. In this article we review techniques used in the investigation of protein interactions with defined nucleosomal templates.
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988, Pubmed,
Xenbase
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988,
Pubmed
,
Xenbase Amir,
Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2.
1999,
Pubmed Becker,
Chromatin assembly extracts from Drosophila embryos.
1994,
Pubmed Cairns,
Chromatin remodeling machines: similar motors, ulterior motives.
1998,
Pubmed Chandler,
Footprinting studies on ligands which stabilize DNA triplexes: effects on stringency within a parallel triple helix.
1995,
Pubmed Chandler,
The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA.
1999,
Pubmed
,
Xenbase Chen,
Modified crush-and-soak method for recovering oligodeoxynucleotides from polyacrylamide gel.
1996,
Pubmed Chevret,
Mini review: form and function in the human interphase chromosome.
2000,
Pubmed Crane-Robinson,
Immunological analysis of chromatin: FIS and CHIPS.
1998,
Pubmed Dixon,
Hydroxyl radical footprinting.
1991,
Pubmed Gelbart,
Interactions of Isw2 chromatin remodeling complex with nucleosomal arrays: analyses using recombinant yeast histones and immobilized templates.
2001,
Pubmed Golding,
Nucleosome structure completely inhibits in vitro cleavage by the V(D)J recombinase.
1999,
Pubmed Guille,
Methods for the analysis of DNA-protein interactions.
1997,
Pubmed Guschin,
SWItched-on mobility.
1999,
Pubmed Guschin,
Asymmetric linker histone association directs the asymmetric rearrangement of core histone interactions in a positioned nucleosome containing a thyroid hormone response element.
1998,
Pubmed
,
Xenbase Hayes,
The histone core exerts a dominant constraint on the structure of DNA in a nucleosome.
1991,
Pubmed Hayes,
Preferential and asymmetric interaction of linker histones with 5S DNA in the nucleosome.
1993,
Pubmed
,
Xenbase Hebbes,
A direct link between core histone acetylation and transcriptionally active chromatin.
1988,
Pubmed Kingston,
ATP-dependent remodeling and acetylation as regulators of chromatin fluidity.
1999,
Pubmed Längst,
Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer.
1999,
Pubmed Li,
Architectural specificity in chromatin structure at the TATA box in vivo: nucleosome displacement upon beta-phaseolin gene activation.
1998,
Pubmed Livingstone-Zatchej,
Mapping of nucleosome positions in yeast.
1999,
Pubmed Noll,
Internal structure of the chromatin subunit.
1974,
Pubmed Pardue,
Heterochromatin: junk or collectors item?
1990,
Pubmed Ramakrishnan,
Histone H1 and chromatin higher-order structure.
1997,
Pubmed Shrader,
Artificial nucleosome positioning sequences.
1989,
Pubmed Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed Thoma,
Local protein-DNA interactions may determine nucleosome positions on yeast plasmids.
,
Pubmed Van Holde,
DNA-histone interactions in nucleosomes.
1980,
Pubmed Varshavsky,
Heterogeneity of chromatin subunits in vitro and location of histone H1.
1976,
Pubmed Vettese-Dadey,
Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores.
1994,
Pubmed Vettese-Dadey,
Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro.
1996,
Pubmed Weintraub,
Chromosomal subunits in active genes have an altered conformation.
1976,
Pubmed Widlund,
Identification and characterization of genomic nucleosome-positioning sequences.
1997,
Pubmed Winston,
Yeast SNF/SWI transcriptional activators and the SPT/SIN chromatin connection.
1992,
Pubmed Wolffe,
The nucleosome: a powerful regulator of transcription.
1998,
Pubmed Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase Zassenhaus,
Rapid electroelution of nucleic acids from agarose and acrylamide gels.
1982,
Pubmed
