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Mol Cell Biol
2006 Feb 01;264:1259-71. doi: 10.1128/MCB.26.4.1259-1271.2006.
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Chromatin decondensation and nuclear reprogramming by nucleoplasmin.
Tamada H, Van Thuan N, Reed P, Nelson D, Katoku-Kikyo N, Wudel J, Wakayama T, Kikyo N.
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Somatic cell nuclear cloning has repeatedly demonstrated striking reversibility of epigenetic regulation of cell differentiation. Upon injection into eggs, the donor nuclei exhibit global chromatin decondensation, which might contribute to reprogramming the nuclei by derepressing dormant genes. Decondensation of sperm chromatin in eggs is explained by the replacement of sperm-specific histone variants with egg-type histones by the egg protein nucleoplasmin (Npm). However, little is known about the mechanisms of chromatin decondensation in somatic nuclei that do not contain condensation-specific histone variants. Here we found that Npm could widely decondense chromatin in undifferentiated mouse cells without overt histone exchanges but with specific epigenetic modifications that are relevant to open chromatin structure. These modifications included nucleus-wide multiple histone H3 phosphorylation, acetylation of Lys 14 in histone H3, and release of heterochromatin proteins HP1beta and TIF1beta from the nuclei. The protein kinase inhibitor staurosporine inhibited chromatin decondensation and these epigenetic modifications with the exception of H3 acetylation, potentially linking these chromatin events. At the functional level, Npm pretreatment of mouse nuclei facilitated activation of four oocyte-specific genes from the nuclei injected into Xenopus laevis oocytes. Future molecular elucidation of chromatin decondensation by Npm will significantly contribute to our understanding of the plasticity of cell differentiation.
Adams,
Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation.
2001, Pubmed
Adams,
Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation.
2001,
Pubmed Ajiro,
Alteration of cell cycle-dependent histone phosphorylations by okadaic acid. Induction of mitosis-specific H3 phosphorylation and chromatin condensation in mammalian interphase cells.
1996,
Pubmed Arnan,
Interaction of nucleoplasmin with core histones.
2003,
Pubmed
,
Xenbase Bannister,
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
2001,
Pubmed Bañuelos,
Activation mechanism of the nuclear chaperone nucleoplasmin: role of the core domain.
2003,
Pubmed
,
Xenbase Barry,
Swelling of hen erythrocyte nuclei in cytoplasm from Xenopus eggs.
1972,
Pubmed
,
Xenbase Blelloch,
Nuclear cloning of embryonal carcinoma cells.
2004,
Pubmed Brero,
Methyl CpG-binding proteins induce large-scale chromatin reorganization during terminal differentiation.
2005,
Pubmed Bürglin,
Cloning of nucleoplasmin from Xenopus laevis oocytes and analysis of its developmental expression.
1987,
Pubmed
,
Xenbase Burns,
Roles of NPM2 in chromatin and nucleolar organization in oocytes and embryos.
2003,
Pubmed
,
Xenbase Byrne,
Nuclei of adult mammalian somatic cells are directly reprogrammed to oct-4 stem cell gene expression by amphibian oocytes.
2003,
Pubmed
,
Xenbase Cammas,
Association of the transcriptional corepressor TIF1beta with heterochromatin protein 1 (HP1): an essential role for progression through differentiation.
2004,
Pubmed Cammas,
Cell differentiation induces TIF1beta association with centromeric heterochromatin via an HP1 interaction.
2002,
Pubmed Carmo-Fonseca,
The contribution of nuclear compartmentalization to gene regulation.
2002,
Pubmed Chen,
Establishment and maintenance of genomic methylation patterns in mouse embryonic stem cells by Dnmt3a and Dnmt3b.
2003,
Pubmed Cheung,
Synergistic coupling of histone H3 phosphorylation and acetylation in response to epidermal growth factor stimulation.
2000,
Pubmed Cheutin,
Maintenance of stable heterochromatin domains by dynamic HP1 binding.
2003,
Pubmed Clayton,
Phosphoacetylation of histone H3 on c-fos- and c-jun-associated nucleosomes upon gene activation.
2000,
Pubmed Cowan,
Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells.
2005,
Pubmed Craig,
Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns.
2003,
Pubmed Dimitrov,
Remodeling somatic nuclei in Xenopus laevis egg extracts: molecular mechanisms for the selective release of histones H1 and H1(0) from chromatin and the acquisition of transcriptional competence.
1996,
Pubmed
,
Xenbase Dutta,
The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly.
2001,
Pubmed Eggan,
Mice cloned from olfactory sensory neurons.
2004,
Pubmed Festenstein,
Modulation of heterochromatin protein 1 dynamics in primary Mammalian cells.
2003,
Pubmed Gonda,
Reversible disassembly of somatic nucleoli by the germ cell proteins FRGY2a and FRGY2b.
2003,
Pubmed
,
Xenbase Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase Gurdon,
Reprogramming of transplanted nuclei in amphibia.
1979,
Pubmed
,
Xenbase Gurdon,
Injected nuclei in frog oocytes:RNA synthesis and protein exchange.
1976,
Pubmed
,
Xenbase Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase Hierro,
Electrostatic interactions at the C-terminal domain of nucleoplasmin modulate its chromatin decondensation activity.
2002,
Pubmed
,
Xenbase Hochedlinger,
Monoclonal mice generated by nuclear transfer from mature B and T donor cells.
2002,
Pubmed Hsu,
Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes.
2000,
Pubmed Hu,
Multilineage gene expression precedes commitment in the hemopoietic system.
1997,
Pubmed Humpherys,
Abnormal gene expression in cloned mice derived from embryonic stem cell and cumulus cell nuclei.
2002,
Pubmed Ito,
ATP-facilitated chromatin assembly with a nucleoplasmin-like protein from Drosophila melanogaster.
1996,
Pubmed
,
Xenbase Jeanisch,
Nuclear cloning, stem cells, and genomic reprogramming.
2002,
Pubmed Kikyo,
Active remodeling of somatic nuclei in egg cytoplasm by the nucleosomal ATPase ISWI.
2000,
Pubmed
,
Xenbase Kipling,
Mouse centromere mapping using oligonucleotide probes that detect variants of the minor satellite.
1994,
Pubmed Lachner,
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
2001,
Pubmed Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase Leno,
Hyperphosphorylation of nucleoplasmin facilitates Xenopus sperm decondensation at fertilization.
1996,
Pubmed
,
Xenbase Lo,
Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14.
2000,
Pubmed Martens,
The profile of repeat-associated histone lysine methylation states in the mouse epigenome.
2005,
Pubmed Marzluff,
The human and mouse replication-dependent histone genes.
2002,
Pubmed Mateescu,
Tethering of HP1 proteins to chromatin is relieved by phosphoacetylation of histone H3.
2004,
Pubmed Mazen,
H3 phosphorylation-dependent structural changes in chromatin. Implications for the role of very lysine-rich histones.
1987,
Pubmed Minc,
Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells.
1999,
Pubmed Miyamoto,
Myeloid or lymphoid promiscuity as a critical step in hematopoietic lineage commitment.
2002,
Pubmed Mullins,
Nuclear transfer in rodents.
2004,
Pubmed Nowak,
Protein phosphatase 2A activity affects histone H3 phosphorylation and transcription in Drosophila melanogaster.
2003,
Pubmed Nowak,
Phosphorylation of histone H3: a balancing act between chromosome condensation and transcriptional activation.
2004,
Pubmed Nowak,
Phosphorylation of histone H3 correlates with transcriptionally active loci.
2000,
Pubmed Ota,
Increased mitotic phosphorylation of histone H3 attributable to AIM-1/Aurora-B overexpression contributes to chromosome number instability.
2002,
Pubmed Peters,
Partitioning and plasticity of repressive histone methylation states in mammalian chromatin.
2003,
Pubmed Phair,
Global nature of dynamic protein-chromatin interactions in vivo: three-dimensional genome scanning and dynamic interaction networks of chromatin proteins.
2004,
Pubmed Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase Philpott,
Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase Prigent,
Phosphorylation of serine 10 in histone H3, what for?
2003,
Pubmed Rice,
Histone methyltransferases direct different degrees of methylation to define distinct chromatin domains.
2003,
Pubmed Rideout,
Generation of mice from wild-type and targeted ES cells by nuclear cloning.
2000,
Pubmed Sealy,
Purification of Xenopus egg nucleoplasmin and its use in chromatin assembly in vitro.
1989,
Pubmed
,
Xenbase Smythe,
Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts.
1991,
Pubmed
,
Xenbase Soloaga,
MSK2 and MSK1 mediate the mitogen- and stress-induced phosphorylation of histone H3 and HMG-14.
2003,
Pubmed Strahl,
The language of covalent histone modifications.
2000,
Pubmed Suemizu,
Expression profiling of placentomegaly associated with nuclear transplantation of mouse ES cells.
2003,
Pubmed Tulin,
Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci.
2003,
Pubmed Verheggen,
Presence of pre-rRNAs before activation of polymerase I transcription in the building process of nucleoli during early development of Xenopus laevis.
1998,
Pubmed
,
Xenbase Wakayama,
Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei.
1998,
Pubmed Wardle,
Refinement of gene expression patterns in the early Xenopus embryo.
2004,
Pubmed
,
Xenbase Wei,
Phosphorylation of histone H3 is required for proper chromosome condensation and segregation.
1999,
Pubmed
