Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Cell
2002 Feb 01;132:454-68. doi: 10.1091/mbc.01-11-0553.
Show Gene links
Show Anatomy links
Activation of p42 mitogen-activated protein kinase (MAPK), but not c-Jun NH(2)-terminal kinase, induces phosphorylation and stabilization of MAPK phosphatase XCL100 in Xenopus oocytes.
???displayArticle.abstract???
Dual-specificity protein phosphatases are implicated in the direct down-regulation of mitogen-activated protein kinase (MAPK) activity in vivo. Accumulating evidence suggests that these phosphatases are components of negative feedback loops that restore MAPK activity to low levels after diverse physiological responses. Limited information exists, however, regarding their posttranscriptional regulation. We cloned two Xenopus homologs of the mammalian dual-specificity MAPK phosphatases MKP-1/CL100 and found that overexpression of XCL100 in G2-arrested oocytes delayed or prevented progesterone-induced meiotic maturation. Epitope-tagged XCL100 was phosphorylated on serine during G2 phase, and on serine and threonine in a p42MAPK-dependent manner during M phase. Threonine phosphorylation mapped to a single residue, threonine 168. Phosphorylation of XCL100 had no measurable effect on its ability to dephosphorylate p42MAPK. Similarly, mutation of threonine 168 to either valine or glutamate did not significantly alter the binding affinity of a catalytically inactive XCL100 protein for active p42MAPK in vivo. XCL100 was a labile protein in G2-arrested and progesterone-stimulated oocytes; surprisingly, its degradation rate was increased more than twofold after exposure to hyperosmolar sorbitol. In sorbitol-treated oocytes expressing a conditionally active DeltaRaf-DD:ER chimera, activation of the p42MAPK cascade led to phosphorylation of XCL100 and a pronounced decrease in the rate of its degradation. Our results provide mechanistic insight into the regulation of a dual-specificity MAPK phosphatase during meiotic maturation and the adaptation to cellular stress.
Abrieu,
MAP kinase does not inactivate, but rather prevents the cyclin degradation pathway from being turned on in Xenopus egg extracts.
1996, Pubmed,
Xenbase
Abrieu,
MAP kinase does not inactivate, but rather prevents the cyclin degradation pathway from being turned on in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Alessi,
The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts.
1993,
Pubmed
,
Xenbase Anderson,
Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
1990,
Pubmed Bagowski,
Bistability in the JNK cascade.
2001,
Pubmed
,
Xenbase Bagowski,
c-Jun N-terminal kinase activation in Xenopus laevis eggs and embryos. A possible non-genomic role for the JNK signaling pathway.
2001,
Pubmed
,
Xenbase Blanco-Aparicio,
A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase.
1999,
Pubmed Bonni,
Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms.
1999,
Pubmed Bosch,
Mutations of critical amino acids affect the biological and biochemical properties of oncogenic A-Raf and Raf-1.
1997,
Pubmed Boyle,
Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates.
1991,
Pubmed Brondello,
Constitutive MAP kinase phosphatase (MKP-1) expression blocks G1 specific gene transcription and S-phase entry in fibroblasts.
1995,
Pubmed Brondello,
The dual specificity mitogen-activated protein kinase phosphatase-1 and -2 are induced by the p42/p44MAPK cascade.
1997,
Pubmed Brondello,
Reduced MAP kinase phosphatase-1 degradation after p42/p44MAPK-dependent phosphorylation.
1999,
Pubmed Camps,
Catalytic activation of the phosphatase MKP-3 by ERK2 mitogen-activated protein kinase.
1998,
Pubmed Charles,
cDNA sequence of a growth factor-inducible immediate early gene and characterization of its encoded protein.
1992,
Pubmed Chen,
Discordance between the binding affinity of mitogen-activated protein kinase subfamily members for MAP kinase phosphatase-2 and their ability to activate the phosphatase catalytically.
2001,
Pubmed Cheng,
Assembly of cyclin D-dependent kinase and titration of p27Kip1 regulated by mitogen-activated protein kinase kinase (MEK1).
1998,
Pubmed Chu,
The mitogen-activated protein kinase phosphatases PAC1, MKP-1, and MKP-2 have unique substrate specificities and reduced activity in vivo toward the ERK2 sevenmaker mutation.
1996,
Pubmed Clark-Lewis,
Definition of a consensus sequence for peptide substrate recognition by p44mpk, the meiosis-activated myelin basic protein kinase.
1991,
Pubmed Corbalan-Garcia,
Identification of the mitogen-activated protein kinase phosphorylation sites on human Sos1 that regulate interaction with Grb2.
1996,
Pubmed Cowley,
Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells.
1994,
Pubmed Crews,
The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.
1992,
Pubmed Cross,
PD 98059 prevents establishment of the spindle assembly checkpoint and inhibits the G2-M transition in meiotic but not mitotic cell cycles in Xenopus.
1998,
Pubmed
,
Xenbase Davis,
Signal transduction by the JNK group of MAP kinases.
2000,
Pubmed Denu,
Form and function in protein dephosphorylation.
1996,
Pubmed Denu,
Visualization of intermediate and transition-state structures in protein-tyrosine phosphatase catalysis.
1996,
Pubmed Dowd,
Isolation of the human genes encoding the pyst1 and Pyst2 phosphatases: characterisation of Pyst2 as a cytosolic dual-specificity MAP kinase phosphatase and its catalytic activation by both MAP and SAP kinases.
1998,
Pubmed Fabian,
Requirement for Raf and MAP kinase function during the meiotic maturation of Xenopus oocytes.
1993,
Pubmed
,
Xenbase Favata,
Identification of a novel inhibitor of mitogen-activated protein kinase kinase.
1998,
Pubmed Ferby,
A novel p34(cdc2)-binding and activating protein that is necessary and sufficient to trigger G(2)/M progression in Xenopus oocytes.
1999,
Pubmed
,
Xenbase Ferrell,
MAP kinases in mitogenesis and development.
1996,
Pubmed
,
Xenbase Ferrell,
Mechanistic studies of the dual phosphorylation of mitogen-activated protein kinase.
1997,
Pubmed
,
Xenbase Fisher,
Dissociation of MAP kinase activation and MPF activation in hormone-stimulated maturation of Xenopus oocytes.
1999,
Pubmed
,
Xenbase Fjeld,
Mechanistic basis for catalytic activation of mitogen-activated protein kinase phosphatase 3 by extracellular signal-regulated kinase.
2000,
Pubmed Furuno,
Suppression of DNA replication via Mos function during meiotic divisions in Xenopus oocytes.
1994,
Pubmed
,
Xenbase Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase Gonzalez,
Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases.
1991,
Pubmed Gotoh,
Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade.
1995,
Pubmed
,
Xenbase Gross,
The critical role of the MAP kinase pathway in meiosis II in Xenopus oocytes is mediated by p90(Rsk).
2000,
Pubmed
,
Xenbase Haycock,
ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ.
1992,
Pubmed Hirsch,
Mitogen-activated protein kinase phosphatases inactivate stress-activated protein kinase pathways in vivo.
1997,
Pubmed Howard,
The mitogen-activated protein kinase signaling pathway stimulates mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation.
1999,
Pubmed
,
Xenbase Hsiao,
Evidence that inactive p42 mitogen-activated protein kinase and inactive Rsk exist as a heterodimer in vivo.
1994,
Pubmed
,
Xenbase Hunter,
Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling.
1995,
Pubmed Ishibashi,
Expression cloning of a human dual-specificity phosphatase.
1992,
Pubmed Izumi,
Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity.
1992,
Pubmed
,
Xenbase Jacobs,
Multiple docking sites on substrate proteins form a modular system that mediates recognition by ERK MAP kinase.
1999,
Pubmed
,
Xenbase Jones,
A Xenopus ribosomal protein S6 kinase has two apparent kinase domains that are each similar to distinct protein kinases.
1988,
Pubmed
,
Xenbase Kamps,
Determination of phosphoamino acid composition by acid hydrolysis of protein blotted to Immobilon.
1991,
Pubmed Keyse,
Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase.
1992,
Pubmed Keyse,
Protein phosphatases and the regulation of MAP kinase activity.
1998,
Pubmed Kosako,
Requirement for the MAP kinase kinase/MAP kinase cascade in Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Kosako,
Xenopus MAP kinase activator is a serine/threonine/tyrosine kinase activated by threonine phosphorylation.
1992,
Pubmed
,
Xenbase Kosako,
Mitogen-activated protein kinase kinase is required for the mos-induced metaphase arrest.
1994,
Pubmed
,
Xenbase Kosako,
Isolation and characterization of neutralizing single-chain antibodies against Xenopus mitogen-activated protein kinase kinase from phage display libraries.
1996,
Pubmed
,
Xenbase Kreegipuu,
PhosphoBase, a database of phosphorylation sites: release 2.0.
1999,
Pubmed Kumagai,
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
1996,
Pubmed
,
Xenbase Lenormand,
Speedy: a novel cell cycle regulator of the G2/M transition.
1999,
Pubmed
,
Xenbase Lewis,
XCL100, an inducible nuclear MAP kinase phosphatase from Xenopus laevis: its role in MAP kinase inactivation in differentiated cells and its expression during early development.
1995,
Pubmed
,
Xenbase Lewis,
Signal transduction through MAP kinase cascades.
1998,
Pubmed Lin,
Premature senescence involving p53 and p16 is activated in response to constitutive MEK/MAPK mitogenic signaling.
1998,
Pubmed Mansour,
Transformation of mammalian cells by constitutively active MAP kinase kinase.
1994,
Pubmed Matten,
Positive feedback between MAP kinase and Mos during Xenopus oocyte maturation.
1996,
Pubmed
,
Xenbase Meier,
Dying like flies.
1998,
Pubmed Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase Muda,
The mitogen-activated protein kinase phosphatase-3 N-terminal noncatalytic region is responsible for tight substrate binding and enzymatic specificity.
1998,
Pubmed Pagès,
Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation.
1993,
Pubmed Palmer,
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1.
1998,
Pubmed
,
Xenbase Payne,
Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).
1991,
Pubmed Pearson,
Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions.
2001,
Pubmed Pfleger,
The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1.
2000,
Pubmed
,
Xenbase Posada,
Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.
1992,
Pubmed
,
Xenbase Robbins,
Regulation and properties of extracellular signal-regulated protein kinases 1 and 2 in vitro.
1993,
Pubmed Rogers,
Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis.
1986,
Pubmed Rohan,
PAC-1: a mitogen-induced nuclear protein tyrosine phosphatase.
1993,
Pubmed Roy,
Mos proto-oncogene function during oocyte maturation in Xenopus.
1996,
Pubmed
,
Xenbase Sagata,
The c-mos proto-oncogene product is a cytostatic factor responsible for meiotic arrest in vertebrate eggs.
1989,
Pubmed
,
Xenbase Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed Shaw,
A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
1986,
Pubmed Slack,
Distinct binding determinants for ERK2/p38alpha and JNK map kinases mediate catalytic activation and substrate selectivity of map kinase phosphatase-1.
2001,
Pubmed Sohaskey,
Distinct, constitutively active MAPK phosphatases function in Xenopus oocytes: implications for p42 MAPK regulation In vivo.
1999,
Pubmed
,
Xenbase Sun,
Inhibition of Ras-induced DNA synthesis by expression of the phosphatase MKP-1.
1994,
Pubmed Sun,
MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo.
1993,
Pubmed Wang,
A role for mitogen-activated protein kinase in the spindle assembly checkpoint in XTC cells.
1997,
Pubmed
,
Xenbase Waskiewicz,
Mitogen and stress response pathways: MAP kinase cascades and phosphatase regulation in mammals and yeast.
1995,
Pubmed Wu,
Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle.
1992,
Pubmed Xia,
Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis.
1995,
Pubmed Yang,
The Elk-1 ETS-domain transcription factor contains a mitogen-activated protein kinase targeting motif.
1998,
Pubmed Yang,
Differential targeting of MAP kinases to the ETS-domain transcription factor Elk-1.
1998,
Pubmed Zhang,
Atomic structure of the MAP kinase ERK2 at 2.3 A resolution.
1994,
Pubmed Zhou,
The catalytic role of Cys124 in the dual specificity phosphatase VHR.
1994,
Pubmed Zhu,
Senescence of human fibroblasts induced by oncogenic Raf.
1998,
Pubmed Zúñiga,
Interaction of mitogen-activated protein kinases with the kinase interaction motif of the tyrosine phosphatase PTP-SL provides substrate specificity and retains ERK2 in the cytoplasm.
1999,
Pubmed
