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Loss of Pax 6 function leads to an eyeless phenotype in both mammals and insects, and ectopic expression of both the Drosophila and the mouse gene leads to the induction of ectopic eyes in Drosophila, which suggested to us that Pax 6 might be a universal master control gene for eye morphogenesis. Here, we report the reciprocal experiment in which the RNAs of the Drosophila Pax 6 homologs, eyeless and twin of eyeless, are transferred into a vertebrate embryo; i.e., early Xenopus embryos at the 2- and 16-cell stages. In both cases, ectopic eye structures are formed. To understand the genetic program specifying eye morphogenesis, we have analyzed the regulatory mechanisms of Pax 6 expression that initiates eye development. Previously, we have demonstrated that Notch signaling regulates the expression of eyeless and twin of eyeless in Drosophila. Here, we show that in Xenopus, activation of Notch signaling also induces eye-related gene expression, including Pax 6, in isolated animal caps. In Xenopus embryos, the activation of Notch signaling causes eye duplications and proximaleye defects, which are also induced by overexpression of eyeless and twin of eyeless. These findings indicate that the gene regulatory cascade is similar in vertebrates and invertebrates.
Altmann,
Lens induction by Pax-6 in Xenopus laevis.
1997, Pubmed,
Xenbase
Altmann,
Lens induction by Pax-6 in Xenopus laevis.
1997,
Pubmed
,
Xenbase Artavanis-Tsakonas,
Notch signaling: cell fate control and signal integration in development.
1999,
Pubmed Bernier,
Expanded retina territory by midbrain transformation upon overexpression of Six6 (Optx2) in Xenopus embryos.
2000,
Pubmed
,
Xenbase Bonini,
The Drosophila eyes absent gene directs ectopic eye formation in a pathway conserved between flies and vertebrates.
1997,
Pubmed Bouwmeester,
Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer.
1996,
Pubmed
,
Xenbase Callaerts,
PAX-6 in development and evolution.
1997,
Pubmed Callaerts,
Isolation and expression of a Pax-6 gene in the regenerating and intact Planarian Dugesia(G)tigrina.
1999,
Pubmed Chen,
Dachshund and eyes absent proteins form a complex and function synergistically to induce ectopic eye development in Drosophila.
1997,
Pubmed Chow,
Pax6 induces ectopic eyes in a vertebrate.
1999,
Pubmed
,
Xenbase Coffman,
Expression of an extracellular deletion of Xotch diverts cell fate in Xenopus embryos.
1993,
Pubmed
,
Xenbase Czerny,
twin of eyeless, a second Pax-6 gene of Drosophila, acts upstream of eyeless in the control of eye development.
1999,
Pubmed Gehring,
The master control gene for morphogenesis and evolution of the eye.
1996,
Pubmed Gehring,
Pax 6: mastering eye morphogenesis and eye evolution.
1999,
Pubmed Glaser,
Genomic structure, evolutionary conservation and aniridia mutations in the human PAX6 gene.
1992,
Pubmed Halder,
New perspectives on eye evolution.
1995,
Pubmed Halder,
Induction of ectopic eyes by targeted expression of the eyeless gene in Drosophila.
1995,
Pubmed Halder,
Eyeless initiates the expression of both sine oculis and eyes absent during Drosophila compound eye development.
1998,
Pubmed Hauck,
Functional analysis of an eye specific enhancer of the eyeless gene in Drosophila.
1999,
Pubmed Hemmati-Brivanlou,
Inhibition of activin receptor signaling promotes neuralization in Xenopus.
1994,
Pubmed
,
Xenbase Hill,
Mouse small eye results from mutations in a paired-like homeobox-containing gene.
,
Pubmed Hirsch,
Xenopus Pax-6 and retinal development.
1997,
Pubmed
,
Xenbase Hollemann,
The Xenopus homologue of the Drosophila gene tailless has a function in early eye development.
1998,
Pubmed
,
Xenbase Jordan,
The human PAX6 gene is mutated in two patients with aniridia.
1992,
Pubmed Kim,
Integration of positional signals and regulation of wing formation and identity by Drosophila vestigial gene.
1996,
Pubmed Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase Kurata,
Notch signaling and the determination of appendage identity.
2000,
Pubmed Li,
A single morphogenetic field gives rise to two retina primordia under the influence of the prechordal plate.
1997,
Pubmed
,
Xenbase Loosli,
Six3, a medaka homologue of the Drosophila homeobox gene sine oculis is expressed in the anterior embryonic shield and the developing eye.
1998,
Pubmed Loosli,
Six3 overexpression initiates the formation of ectopic retina.
1999,
Pubmed Mathers,
Regulation of eye formation by the Rx and pax6 homeobox genes.
2000,
Pubmed Mathers,
The Rx homeobox gene is essential for vertebrate eye development.
1997,
Pubmed
,
Xenbase Niimi,
Direct regulatory interaction of the eyeless protein with an eye-specific enhancer in the sine oculis gene during eye induction in Drosophila.
1999,
Pubmed Oliver,
Ectopic lens induction in fish in response to the murine homeobox gene Six3.
1996,
Pubmed
,
Xenbase Pignoni,
The eye-specification proteins So and Eya form a complex and regulate multiple steps in Drosophila eye development.
1997,
Pubmed Pineda,
Searching for the prototypic eye genetic network: Sine oculis is essential for eye regeneration in planarians.
2000,
Pubmed Punzo,
The eyeless homeodomain is dispensable for eye development in Drosophila.
2001,
Pubmed Quiring,
Homology of the eyeless gene of Drosophila to the Small eye gene in mice and Aniridia in humans.
1994,
Pubmed Schneuwly,
Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia.
,
Pubmed Seimiya,
The Drosophila homeobox gene optix is capable of inducing ectopic eyes by an eyeless-independent mechanism.
2000,
Pubmed Shen,
Ectopic eye development in Drosophila induced by directed dachshund expression.
1997,
Pubmed Struhl,
Genes controlling segmental specification in the Drosophila thorax.
1982,
Pubmed Ton,
Positional cloning and characterization of a paired box- and homeobox-containing gene from the aniridia region.
1991,
Pubmed Walther,
Pax-6, a murine paired box gene, is expressed in the developing CNS.
1991,
Pubmed Xu,
Regulation of Pax6 expression is conserved between mice and flies.
1999,
Pubmed Younossi-Hartenstein,
Embryonic origin of the imaginal discs of the head of Drosophila melanogaster.
1993,
Pubmed
