Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2002 Feb 01;213:365-76. doi: 10.1093/emboj/21.3.365.
Show Gene links
Show Anatomy links
Krox20 and kreisler co-operate in the transcriptional control of segmental expression of Hoxb3 in the developing hindbrain.
Manzanares M, Nardelli J, Gilardi-Hebenstreit P, Marshall H, Giudicelli F, Martínez-Pastor MT, Krumlauf R, Charnay P.
???displayArticle.abstract???
In the segmented vertebrate hindbrain, the Hoxa3 and Hoxb3 genes are expressed at high relative levels in the rhombomeres (r) 5 and 6, and 5, respectively. The single enhancer elements responsible for these activities have been identified previously and shown to constitute direct targets of the transcription factor kreisler, which is expressed in r5 and r6. Here, we have analysed the contribution of the transcription factor Krox20, present in r3 and r5. Genetic analyses demonstrated that Krox20 is required for activity of the Hoxb3r5 enhancer, but not of the Hoxa3r5/6 enhancer. Mutational analysis of the Hoxb3r5 enhancer, together with ectopic expression experiments, revealed that Krox20 binds to the enhancer and synergizes with kreisler to promote Hoxb3 transcription, restricting enhancer activity to their domain of overlap, r5. These analyses also suggested contributions from an Ets-related factor and from putative factors likely to heterodimerize with kreisler. The integration of multiple independent inputs present in overlapping domains by a single enhancer is likely to constitute a general mechanism for the patterning of subterritories during vertebrate development.
Affolter,
Development. Legs, eyes, or wings--selectors and signals make the difference.
2001, Pubmed
Affolter,
Development. Legs, eyes, or wings--selectors and signals make the difference.
2001,
Pubmed Blank,
The Maf transcription factors: regulators of differentiation.
1997,
Pubmed Bosselut,
The product of the c-ets-1 proto-oncogene and the related Ets2 protein act as transcriptional activators of the long terminal repeat of human T cell leukemia virus HTLV-1.
1990,
Pubmed Carpenter,
Loss of Hox-A1 (Hox-1.6) function results in the reorganization of the murine hindbrain.
1993,
Pubmed Chavrier,
The segment-specific gene Krox-20 encodes a transcription factor with binding sites in the promoter region of the Hox-1.4 gene.
1990,
Pubmed Chen,
An enhancer element in the EphA2 (Eck) gene sufficient for rhombomere-specific expression is activated by HOXA1 and HOXB1 homeobox proteins.
1998,
Pubmed Cordes,
The mouse segmentation gene kr encodes a novel basic domain-leucine zipper transcription factor.
1994,
Pubmed Davenne,
Hoxa2 and Hoxb2 control dorsoventral patterns of neuronal development in the rostral hindbrain.
1999,
Pubmed Dollé,
Local alterations of Krox-20 and Hox gene expression in the hindbrain suggest lack of rhombomeres 4 and 5 in homozygote null Hoxa-1 (Hox-1.6) mutant embryos.
1993,
Pubmed Frohman,
Altered rhombomere-specific gene expression and hyoid bone differentiation in the mouse segmentation mutant, kreisler (kr).
1993,
Pubmed Gavalas,
Role of Hoxa-2 in axon pathfinding and rostral hindbrain patterning.
1997,
Pubmed Gavalas,
Hoxa1 and Hoxb1 synergize in patterning the hindbrain, cranial nerves and second pharyngeal arch.
1998,
Pubmed Gavalas,
Synergy between Hoxa1 and Hoxb1: the relationship between arch patterning and the generation of cranial neural crest.
2001,
Pubmed Giudicelli,
Krox-20 patterns the hindbrain through both cell-autonomous and non cell-autonomous mechanisms.
2001,
Pubmed Godsave,
Expression patterns of Hoxb genes in the Xenopus embryo suggest roles in anteroposterior specification of the hindbrain and in dorsoventral patterning of the mesoderm.
1994,
Pubmed
,
Xenbase Helmbacher,
Hoxa1 and Krox-20 synergize to control the development of rhombomere 3.
1998,
Pubmed Hunt,
A distinct Hox code for the branchial region of the vertebrate head.
1991,
Pubmed Itasaki,
'Shocking' developments in chick embryology: electroporation and in ovo gene expression.
1999,
Pubmed Kataoka,
A set of Hox proteins interact with the Maf oncoprotein to inhibit its DNA binding, transactivation, and transforming activities.
2001,
Pubmed Krumlauf,
Hox genes in vertebrate development.
1994,
Pubmed Lumsden,
Patterning the vertebrate neuraxis.
1996,
Pubmed Lumsden,
The cellular basis of segmentation in the developing hindbrain.
1990,
Pubmed Maconochie,
Differences in Krox20-dependent regulation of Hoxa2 and Hoxb2 during hindbrain development.
2001,
Pubmed Maconochie,
Paralogous Hox genes: function and regulation.
1996,
Pubmed Mann,
Hox proteins meet more partners.
1998,
Pubmed Manzanares,
Conserved and distinct roles of kreisler in regulation of the paralogous Hoxa3 and Hoxb3 genes.
1999,
Pubmed
,
Xenbase Manzanares,
The role of kreisler in segmentation during hindbrain development.
1999,
Pubmed Manzanares,
Independent regulation of initiation and maintenance phases of Hoxa3 expression in the vertebrate hindbrain involve auto- and cross-regulatory mechanisms.
2001,
Pubmed
,
Xenbase Manzanares,
Segmental regulation of Hoxb-3 by kreisler.
1997,
Pubmed
,
Xenbase Mark,
Two rhombomeres are altered in Hoxa-1 mutant mice.
1993,
Pubmed McKay,
The kreisler mouse: a hindbrain segmentation mutant that lacks two rhombomeres.
1994,
Pubmed Mechta-Grigoriou,
Nab proteins mediate a negative feedback loop controlling Krox-20 activity in the developing hindbrain.
2000,
Pubmed Moens,
Equivalence in the genetic control of hindbrain segmentation in fish and mouse.
1998,
Pubmed Moens,
valentino: a zebrafish gene required for normal hindbrain segmentation.
1996,
Pubmed Nardelli,
Base sequence discrimination by zinc-finger DNA-binding domains.
1991,
Pubmed Nieto,
Conserved segmental expression of Krox-20 in the vertebrate hindbrain and its relationship to lineage restriction.
1991,
Pubmed
,
Xenbase Nonchev,
Segmental expression of Hoxa-2 in the hindbrain is directly regulated by Krox-20.
1996,
Pubmed Nonchev,
The conserved role of Krox-20 in directing Hox gene expression during vertebrate hindbrain segmentation.
1996,
Pubmed Prince,
Zebrafish hox genes: expression in the hindbrain region of wild-type and mutants of the segmentation gene, valentino.
1998,
Pubmed Ruiz i Altaba,
Pattern formation in the vertebrate neural plate.
1994,
Pubmed Schneider-Maunoury,
Segmental and neuronal architecture of the hindbrain of Krox-20 mouse mutants.
1997,
Pubmed Schneider-Maunoury,
How to build a vertebrate hindbrain. Lessons from genetics.
1998,
Pubmed Schneider-Maunoury,
Disruption of Krox-20 results in alteration of rhombomeres 3 and 5 in the developing hindbrain.
1993,
Pubmed Seitanidou,
Krox-20 is a key regulator of rhombomere-specific gene expression in the developing hindbrain.
1997,
Pubmed Sham,
The zinc finger gene Krox20 regulates HoxB2 (Hox2.8) during hindbrain segmentation.
1993,
Pubmed Sieweke,
MafB is an interaction partner and repressor of Ets-1 that inhibits erythroid differentiation.
1996,
Pubmed Studer,
Genetic interactions between Hoxa1 and Hoxb1 reveal new roles in regulation of early hindbrain patterning.
1998,
Pubmed Swiatek,
Perinatal lethality and defects in hindbrain development in mice homozygous for a targeted mutation of the zinc finger gene Krox20.
1993,
Pubmed Swirnoff,
DNA-binding specificity of NGFI-A and related zinc finger transcription factors.
1995,
Pubmed Theil,
Requirement for downregulation of kreisler during late patterning of the hindbrain.
2002,
Pubmed Theil,
Segmental expression of the EphA4 (Sek-1) receptor tyrosine kinase in the hindbrain is under direct transcriptional control of Krox-20.
1998,
Pubmed Trainor,
Genetic interactions during hindbrain segmentation in the mouse embryo.
2000,
Pubmed Trainor,
Patterning the cranial neural crest: hindbrain segmentation and Hox gene plasticity.
2000,
Pubmed Vesque,
Hoxb-2 transcriptional activation in rhombomeres 3 and 5 requires an evolutionarily conserved cis-acting element in addition to the Krox-20 binding site.
1996,
Pubmed Voiculescu,
Hindbrain patterning: Krox20 couples segmentation and specification of regional identity.
2001,
Pubmed Whiting,
Multiple spatially specific enhancers are required to reconstruct the pattern of Hox-2.6 gene expression.
1991,
Pubmed Wilkinson,
Segment-specific expression of a zinc-finger gene in the developing nervous system of the mouse.
1989,
Pubmed Yee,
The regulation of myogenin gene expression during the embryonic development of the mouse.
1993,
Pubmed
