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Translational control is a key level in regulating gene expression in oocytes and eggs because many mRNAs are synthesized and stored during oogenesis for latter use at various stages of oocyte maturation and embryonic development. Understanding the molecular mechanisms that underlie this translational control is therefore crucial. Another important issue is the evolutionary conservation of these mechanisms--in other words the determination of their universal and specific aspects. We report here a comparative analysis of a translational repression mechanism that depends on the EDEN (embryo deadenylation element) element. This small cis-acting element, localized in the 3' untranslated region of c-mos and Eg mRNAs, was shown to be involved in a deadenylation process. We demonstrate here that in Xenopus embryos, mRNAs that contain an EDEN are translationally repressed. Next, transgenic flies were used to study the effect of the EDEN motif on translation in Drosophila oocytes. We show that this element also causes the translational repression of a reporter gene in Drosophila demonstrating that the EDEN-dependent translational repression is functionally conserved between Xenopus and Drosophila.
Aït-Ahmed,
The yemanuclein-alpha: a new Drosophila DNA binding protein specific for the oocyte nucleus.
1992, Pubmed
Aït-Ahmed,
The yemanuclein-alpha: a new Drosophila DNA binding protein specific for the oocyte nucleus.
1992,
Pubmed Audic,
Embryo deadenylation element-dependent deadenylation is enhanced by a cis element containing AUU repeats.
1998,
Pubmed
,
Xenbase Bouvet,
The deadenylation conferred by the 3' untranslated region of a developmentally controlled mRNA in Xenopus embryos is switched to polyadenylation by deletion of a short sequence element.
1994,
Pubmed
,
Xenbase Capri,
Implication of a 5' coding sequence in targeting maternal mRNA to the Drosophila oocyte.
1997,
Pubmed Chagnovich,
Poly(A)-independent regulation of maternal hunchback translation in the Drosophila embryo.
2001,
Pubmed Curtis,
Translational regulation in development.
1995,
Pubmed Gebauer,
Translational control of dosage compensation in Drosophila by Sex-lethal: cooperative silencing via the 5' and 3' UTRs of msl-2 mRNA is independent of the poly(A) tail.
1999,
Pubmed Huarte,
Transient translational silencing by reversible mRNA deadenylation.
1992,
Pubmed Kim-Ha,
Translational regulation of oskar mRNA by bruno, an ovarian RNA-binding protein, is essential.
1995,
Pubmed Lie,
Translational regulation of oskar mRNA occurs independent of the cap and poly(A) tail in Drosophila ovarian extracts.
1999,
Pubmed Paillard,
EDEN and EDEN-BP, a cis element and an associated factor that mediate sequence-specific mRNA deadenylation in Xenopus embryos.
1998,
Pubmed
,
Xenbase Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase Paris,
Changes in the polyadenylation of specific stable RNA during the early development of Xenopus laevis.
1988,
Pubmed
,
Xenbase Richter,
Cytoplasmic polyadenylation in development and beyond.
1999,
Pubmed
,
Xenbase Rio,
Transformation of cultured Drosophila melanogaster cells with a dominant selectable marker.
1985,
Pubmed Robbie,
Temporal regulation of the Xenopus FGF receptor in development: a translation inhibitory element in the 3' untranslated region.
1995,
Pubmed
,
Xenbase Sallés,
Coordinate initiation of Drosophila development by regulated polyadenylation of maternal messenger RNAs.
1994,
Pubmed Sanes,
Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos.
1986,
Pubmed Santoni,
A sequence based computational identification of a Drosophila developmentally regulated TATA-less RNA polymerase II promoter and its experimental validation.
1998,
Pubmed Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase Sleigh,
A nonchromatographic assay for expression of the chloramphenicol acetyltransferase gene in eucaryotic cells.
1986,
Pubmed Tautz,
A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.
1989,
Pubmed Thompson,
Rapid deadenylation and Poly(A)-dependent translational repression mediated by the Caenorhabditis elegans tra-2 3' untranslated region in Xenopus embryos.
2000,
Pubmed
,
Xenbase Verrotti,
Evolutionary conservation of sequence elements controlling cytoplasmic polyadenylylation.
1996,
Pubmed
,
Xenbase Webster,
Translational repressor bruno plays multiple roles in development and is widely conserved.
1997,
Pubmed
