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Gating properties of Na(v)1.7 and Na(v)1.8 peripheral nerve sodium channels.
Vijayaragavan K, O'Leary ME, Chahine M.
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Several distinct components of voltage-gated sodium current have been recorded from native dorsal root ganglion (DRG) neurons that display differences in gating and pharmacology. This study compares the electrophysiological properties of two peripheral nerve sodium channels that are expressed selectively in DRG neurons (Na(v)1.7 and Na(v)1.8). Recombinant Na(v)1.7 and Na(v)1.8 sodium channels were coexpressed with the auxiliary beta(1) subunit in Xenopus oocytes. In this system coexpression of the beta(1) subunit with Na(v)1.7 and Na(v)1.8 channels results in more rapid inactivation, a shift in midpoints of steady-state activation and inactivation to more hyperpolarizing potentials, and an acceleration of recovery from inactivation. The coinjection of beta(1) subunit also significantly increases the expression of Na(v)1.8 by sixfold but has no effect on the expression of Na(v)1.7. In addition, a great percentage of Na(v)1.8+beta(1) channels is observed to enter rapidly into the slow inactivated states, in contrast to Nav1.7+beta(1) channels. Consequently, the rapid entry into slow inactivation is believed to cause a frequency-dependent reduction of Na(v)1.8+beta(1) channel amplitudes, seen during repetitive pulsing between 1 and 2 Hz. However, at higher frequencies (>20 Hz) Na(v)1.8+beta(1) channels reach a steady state to approximately 42% of total current. The presence of this steady-state sodium channel activity, coupled with the high activation threshold (V(0.5) = -3.3 mV) of Na(v)1.8+beta(1), could enable the nociceptive fibers to fire spontaneously after nerve injury.
Akopian,
A tetrodotoxin-resistant voltage-gated sodium channel expressed by sensory neurons.
1996, Pubmed,
Xenbase
Akopian,
A tetrodotoxin-resistant voltage-gated sodium channel expressed by sensory neurons.
1996,
Pubmed
,
Xenbase Arbuckle,
Expression of tetrodotoxin-resistant sodium channels in capsaicin-sensitive dorsal root ganglion neurons of adult rats.
1995,
Pubmed Auld,
A rat brain Na+ channel alpha subunit with novel gating properties.
1988,
Pubmed
,
Xenbase Bennett,
A molecular basis for gating mode transitions in human skeletal muscle Na+ channels.
1993,
Pubmed
,
Xenbase Black,
Spinal sensory neurons express multiple sodium channel alpha-subunit mRNAs.
1996,
Pubmed Blackburn-Munro,
The sodium channel auxiliary subunits beta1 and beta2 are differentially expressed in the spinal cord of neuropathic rats.
1999,
Pubmed Caffrey,
Three types of sodium channels in adult rat dorsal root ganglion neurons.
1992,
Pubmed Caldwell,
Clustering of sodium channels at the neuromuscular junction.
2000,
Pubmed Chahine,
Functional expression and properties of the human skeletal muscle sodium channel.
1994,
Pubmed
,
Xenbase Coward,
Sodium channel beta1 and beta2 subunits parallel SNS/PN3 alpha-subunit changes in injured human sensory neurons.
2001,
Pubmed Cummins,
Downregulation of tetrodotoxin-resistant sodium currents and upregulation of a rapidly repriming tetrodotoxin-sensitive sodium current in small spinal sensory neurons after nerve injury.
1997,
Pubmed Dib-Hajj,
NaN, a novel voltage-gated Na channel, is expressed preferentially in peripheral sensory neurons and down-regulated after axotomy.
1998,
Pubmed Elliott,
Characterization of TTX-sensitive and TTX-resistant sodium currents in small cells from adult rat dorsal root ganglia.
1993,
Pubmed Gold,
Tetrodotoxin-resistant Na+ currents and inflammatory hyperalgesia.
1999,
Pubmed Gold,
Hyperalgesic agents increase a tetrodotoxin-resistant Na+ current in nociceptors.
1996,
Pubmed Goldin,
Nomenclature of voltage-gated sodium channels.
2000,
Pubmed Goldin,
Messenger RNA coding for only the alpha subunit of the rat brain Na channel is sufficient for expression of functional channels in Xenopus oocytes.
1986,
Pubmed
,
Xenbase Harper,
Electrical properties of rat dorsal root ganglion neurones with different peripheral nerve conduction velocities.
1985,
Pubmed Isom,
Primary structure and functional expression of the beta 1 subunit of the rat brain sodium channel.
1992,
Pubmed
,
Xenbase Isom,
Functional co-expression of the beta 1 and type IIA alpha subunits of sodium channels in a mammalian cell line.
1995,
Pubmed
,
Xenbase Jeftinija,
The role of tetrodotoxin-resistant sodium channels of small primary afferent fibers.
1994,
Pubmed Kostyuk,
Ionic currents in the somatic membrane of rat dorsal root ganglion neurons-I. Sodium currents.
1981,
Pubmed Makielski,
Coexpression of beta 1 with cardiac sodium channel alpha subunits in oocytes decreases lidocaine block.
1996,
Pubmed
,
Xenbase Makita,
Voltage-gated Na+ channel beta 1 subunit mRNA expressed in adult human skeletal muscle, heart, and brain is encoded by a single gene.
1994,
Pubmed
,
Xenbase McClatchey,
The cloning and expression of a sodium channel beta 1-subunit cDNA from human brain.
1993,
Pubmed
,
Xenbase MeLean,
Subtypes of dorsal root ganglion neurons based on different inward currents as measured by whole-cell voltage clamp.
1988,
Pubmed Morgan,
beta 3: an additional auxiliary subunit of the voltage-sensitive sodium channel that modulates channel gating with distinct kinetics.
2000,
Pubmed
,
Xenbase Novakovic,
Distribution of the tetrodotoxin-resistant sodium channel PN3 in rat sensory neurons in normal and neuropathic conditions.
1998,
Pubmed Nuss,
Functional association of the beta 1 subunit with human cardiac (hH1) and rat skeletal muscle (mu 1) sodium channel alpha subunits expressed in Xenopus oocytes.
1995,
Pubmed
,
Xenbase Ogata,
Slow inactivation of tetrodotoxin-insensitive Na+ channels in neurons of rat dorsal root ganglia.
1992,
Pubmed Ogata,
Kinetic analysis of two types of Na+ channels in rat dorsal root ganglia.
1993,
Pubmed Oh,
Na+ channel beta 1 subunit mRNA: differential expression in rat spinal sensory neurons.
1995,
Pubmed O'Leary,
Characterization of the isoform-specific differences in the gating of neuronal and muscle sodium channels.
1998,
Pubmed Porreca,
A comparison of the potential role of the tetrodotoxin-insensitive sodium channels, PN3/SNS and NaN/SNS2, in rat models of chronic pain.
1999,
Pubmed Rizzo,
Selective loss of slow and enhancement of fast Na+ currents in cutaneous afferent dorsal root ganglion neurones following axotomy.
1995,
Pubmed Roy,
Differential properties of tetrodotoxin-sensitive and tetrodotoxin-resistant sodium channels in rat dorsal root ganglion neurons.
1992,
Pubmed Rush,
Electrophysiological properties of sodium current subtypes in small cells from adult rat dorsal root ganglia.
1998,
Pubmed Sangameswaran,
A novel tetrodotoxin-sensitive, voltage-gated sodium channel expressed in rat and human dorsal root ganglia.
1997,
Pubmed
,
Xenbase Sangameswaran,
Structure and function of a novel voltage-gated, tetrodotoxin-resistant sodium channel specific to sensory neurons.
1996,
Pubmed
,
Xenbase Schild,
Experimental and modeling study of Na+ current heterogeneity in rat nodose neurons and its impact on neuronal discharge.
1997,
Pubmed Scholz,
Complex blockade of TTX-resistant Na+ currents by lidocaine and bupivacaine reduce firing frequency in DRG neurons.
1998,
Pubmed Shah,
beta3, a novel auxiliary subunit for the voltage-gated sodium channel, is expressed preferentially in sensory neurons and is upregulated in the chronic constriction injury model of neuropathic pain.
2000,
Pubmed
,
Xenbase Shcherbatko,
Voltage-dependent sodium channel function is regulated through membrane mechanics.
1999,
Pubmed
,
Xenbase Toledo-Aral,
Identification of PN1, a predominant voltage-dependent sodium channel expressed principally in peripheral neurons.
1997,
Pubmed Wallner,
Modulation of the skeletal muscle sodium channel alpha-subunit by the beta 1-subunit.
1993,
Pubmed
,
Xenbase Yang,
Expression of the sodium channel beta 1 subunit in rat skeletal muscle is selectively associated with the tetrodotoxin-sensitive alpha subunit isoform.
1993,
Pubmed
