Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2001 Aug 15;2016:4467-77. doi: 10.1093/emboj/20.16.4467.
Show Gene links
Show Anatomy links
Identification of a mammalian H(+)-myo-inositol symporter expressed predominantly in the brain.
Uldry M, Ibberson M, Horisberger JD, Chatton JY, Riederer BM, Thorens B.
???displayArticle.abstract???
Inositol and its phosphorylated derivatives play a major role in brain function, either as osmolytes, second messengers or regulators of vesicle endo- and exocytosis. Here we describe the identification and functional characterization of a novel H(+)-myo- inositol co-transporter, HMIT, expressed predominantly in the brain. HMIT cDNA encodes a 618 amino acid polypeptide with 12 predicted transmembrane domains. Functional expression of HMIT in Xenopus oocytes showed that transport activity was specific for myo-inositol and related stereoisomers with a Michaelis-Menten constant of approximately 100 microM, and that transport activity was strongly stimulated by decreasing pH. Electrophysiological measurements revealed that transport was electrogenic with a maximal transport activity reached at pH 5.0. In rat brain membrane preparations, HMIT appeared as a 75-90 kDa protein that could be converted to a 67 kDa band upon enzymatic deglycosylation. Immunofluorescence microscopy analysis showed HMIT expression in glial cells and some neurons. These data provide the first characterization of a mammalian H(+)-coupled myo- inositol transporter. Predominant central expression of HMIT suggests that it has a key role in the control of myo-inositol brain metabolism.
Atwal,
The TrkB-Shc site signals neuronal survival and local axon growth via MEK and P13-kinase.
2000, Pubmed
Atwal,
The TrkB-Shc site signals neuronal survival and local axon growth via MEK and P13-kinase.
2000,
Pubmed Babinski,
Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+.
2000,
Pubmed
,
Xenbase Bassilana,
The acid-sensitive ionic channel subunit ASIC and the mammalian degenerin MDEG form a heteromultimeric H+-gated Na+ channel with novel properties.
1997,
Pubmed
,
Xenbase Berridge,
Neural and developmental actions of lithium: a unifying hypothesis.
1989,
Pubmed Berry,
In vivo brain myo-inositol levels in children with Down syndrome.
1999,
Pubmed Brown,
Structure of the novel membrane-coating material in proton-secreting epithelial cells and identification as an H+ATPase.
1987,
Pubmed Chatton,
Modulation of hormone-induced calcium oscillations by intracellular pH in rat hepatocytes.
1997,
Pubmed Chatton,
A quantitative analysis of L-glutamate-regulated Na+ dynamics in mouse cortical astrocytes: implications for cellular bioenergetics.
2000,
Pubmed Cheatham,
Insulin action and the insulin signaling network.
1995,
Pubmed Chesler,
Modulation of pH by neuronal activity.
1992,
Pubmed Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed Cicero,
A sensitive and specific method for the measurement of monophosphoinositide at a microregional level in brain.
1973,
Pubmed Cremona,
Essential role of phosphoinositide metabolism in synaptic vesicle recycling.
1999,
Pubmed Gaidarov,
A functional phosphatidylinositol 3,4,5-trisphosphate/phosphoinositide binding domain in the clathrin adaptor AP-2 alpha subunit. Implications for the endocytic pathway.
1996,
Pubmed Geering,
Oligomerization and maturation of Na,K-ATPase: functional interaction of the cytoplasmic NH2 terminus of the beta subunit with the alpha subunit.
1996,
Pubmed
,
Xenbase Hediger,
Molecular physiology of sodium-glucose cotransporters.
1994,
Pubmed Ibberson,
GLUTX1, a novel mammalian glucose transporter expressed in the central nervous system and insulin-sensitive tissues.
2000,
Pubmed
,
Xenbase Irvine,
Inositol phospholipids: translocation, translocation, translocation..
,
Pubmed Jost,
Phosphatidylinositol-4,5-bisphosphate is required for endocytic coated vesicle formation.
,
Pubmed Kaplan,
Neurotrophin signal transduction in the nervous system.
2000,
Pubmed Kwon,
Cloning of the cDNa for a Na+/myo-inositol cotransporter, a hypertonicity stress protein.
1992,
Pubmed
,
Xenbase Leevers,
Signalling through phosphoinositide 3-kinases: the lipids take centre stage.
1999,
Pubmed Matskevitch,
Effect of extracellular pH on the myo-inositol transporter SMIT expressed in Xenopus oocytes.
1998,
Pubmed
,
Xenbase Mehrotra,
Binding kinetics and ligand specificity for the interactions of the C2B domain of synaptogmin II with inositol polyphosphates and phosphoinositides.
2000,
Pubmed Miyakawa,
Tonicity-responsive enhancer binding protein, a rel-like protein that stimulates transcription in response to hypertonicity.
1999,
Pubmed Ohara-Imaizumi,
Distinct roles of C2A and C2B domains of synaptotagmin in the regulation of exocytosis in adrenal chromaffin cells.
1997,
Pubmed Rapoport,
Regulatory interactions in the recognition of endocytic sorting signals by AP-2 complexes.
1997,
Pubmed Schiavo,
A possible docking and fusion particle for synaptic transmission.
1995,
Pubmed Sherman,
The measurement of myo-inositol, myo-inosose-2 and scyllo-inositol in mammalian tissues.
1968,
Pubmed Shetty,
Polyol profiles in Down syndrome. myo-Inositol, specifically, is elevated in the cerebrospinal fluid.
1995,
Pubmed Simonsen,
EEA1 links PI(3)K function to Rab5 regulation of endosome fusion.
1998,
Pubmed Spector,
The specificity and sulfhydryl sensitivity of the inositol transport system of the central nervous system.
1976,
Pubmed Thomas,
Experimental displacement of intracellular pH and the mechanism of its subsequent recovery.
1984,
Pubmed Tokuyasu,
A study of positive staining of ultrathin frozen sections.
1978,
Pubmed Waldmann,
A proton-gated cation channel involved in acid-sensing.
1997,
Pubmed
,
Xenbase Wang,
A conformation of Na(+)-K+ pump is permeable to proton.
1995,
Pubmed
,
Xenbase Widmann,
Agonist-induced internalization and recycling of the glucagon-like peptide-1 receptor in transfected fibroblasts and in insulinomas.
1995,
Pubmed Williams,
Lithium therapy and signal transduction.
2000,
Pubmed Zagon,
Spectrin subtypes in mammalian brain: an immunoelectron microscopic study.
1986,
Pubmed Zerangue,
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
1999,
Pubmed
,
Xenbase
