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Proc Natl Acad Sci U S A
2001 Jul 31;9816:9425-30. doi: 10.1073/pnas.141241098.
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Identification of a chloride-formate exchanger expressed on the brush border membrane of renal proximal tubule cells.
Knauf F, Yang CL, Thomson RB, Mentone SA, Giebisch G, Aronson PS.
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A key function of the proximal tubule is retrieval of most of the vast quantities of NaCl and water filtered by the kidney. Physiological studies using brush border vesicles and perfused tubules have indicated that a major fraction of Cl(-) reabsorption across the apical membrane of proximal tubule cells occurs via Cl(-)-formate exchange. The molecular identity of the transporter responsible for renal brush border Cl(-)-formate exchange has yet to be elucidated. As a strategy to identify one or more anion exchangers responsible for mediating Cl(-) reabsorption in the proximal tubule, we screened the expressed sequence tag database for homologs of pendrin, a transporter previously shown to mediate Cl(-)-formate exchange. We now report the cDNA cloning of CFEX, a mouse pendrin homolog with expression in the kidney by Northern analysis. Sequence analysis indicated that CFEX very likely represents the mouse ortholog of human SLC26A6. Immunolocalization studies detected expression of CFEX, but not pendrin, on the brush border membrane of proximal tubule cells. Functional expression studies in Xenopus oocytes demonstrated that CFEX mediates Cl(-)-formate exchange. Taken together, these observations identify CFEX as a prime candidate to mediate Cl(-)-formate exchange in the proximal tubule and thereby to contribute importantly to renal NaCl reabsorption. Given its wide tissue distribution, CFEX also may contribute to transcellular Cl(-) transport in additional epithelia such as the pancreas and contribute to transmembrane Cl(-) transport in nonepithelial tissues such as the heart.
Amemiya,
Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb.
1995, Pubmed
Amemiya,
Expression of NHE-3 in the apical membrane of rat renal proximal tubule and thick ascending limb.
1995,
Pubmed Aronson,
Mechanisms of chloride transport in the proximal tubule.
1997,
Pubmed Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983,
Pubmed
,
Xenbase Biemesderfer,
NHE3: a Na+/H+ exchanger isoform of renal brush border.
1993,
Pubmed Biemesderfer,
Active (9.6 s) and inactive (21 s) oligomers of NHE3 in microdomains of the renal brush border.
2001,
Pubmed Bissig,
Functional expression cloning of the canalicular sulfate transport system of rat hepatocytes.
1994,
Pubmed
,
Xenbase Everett,
Pendred syndrome is caused by mutations in a putative sulphate transporter gene (PDS).
1997,
Pubmed Hästbacka,
The diastrophic dysplasia gene encodes a novel sulfate transporter: positional cloning by fine-structure linkage disequilibrium mapping.
1994,
Pubmed Höglund,
Mutations of the Down-regulated in adenoma (DRA) gene cause congenital chloride diarrhoea.
1996,
Pubmed Karniski,
Anion exchange pathways for Cl- transport in rabbit renal microvillus membranes.
1987,
Pubmed Karniski,
Chloride/formate exchange with formic acid recycling: a mechanism of active chloride transport across epithelial membranes.
1985,
Pubmed Kuo,
Pathways for oxalate transport in rabbit renal microvillus membrane vesicles.
1996,
Pubmed Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed Larsson,
The ultrastructure of the developing proximal tubule in the rat kidney.
1975,
Pubmed Lohi,
Mapping of five new putative anion transporter genes in human and characterization of SLC26A6, a candidate gene for pancreatic anion exchanger.
2000,
Pubmed Maren,
Carbon dioxide equilibria in the kidney: the problems of elevated carbon dioxide tension, delayed dehydration, and disequilibrium pH.
1978,
Pubmed McConnell,
Effects of inhibitors on anion exchangers in rabbit renal brush border membrane vesicles.
1994,
Pubmed Royaux,
Pendrin, encoded by the Pendred syndrome gene, resides in the apical region of renal intercalated cells and mediates bicarbonate secretion.
2001,
Pubmed Schild,
Effect of formate on volume reabsorption in the rabbit proximal tubule.
1987,
Pubmed Schultheis,
Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger.
1998,
Pubmed Scott,
The Pendred syndrome gene encodes a chloride-iodide transport protein.
1999,
Pubmed
,
Xenbase Scott,
Human pendrin expressed in Xenopus laevis oocytes mediates chloride/formate exchange.
2000,
Pubmed
,
Xenbase Sheu,
Heterogeneity of chloride/base exchange in rabbit superficial and juxtamedullary proximal convoluted tubules.
1995,
Pubmed Soleimani,
Pendrin: an apical Cl-/OH-/HCO3- exchanger in the kidney cortex.
2001,
Pubmed Soleimani,
Presence of chloride-formate exchange in vascular smooth muscle and cardiac cells.
1994,
Pubmed Tokuyasu,
Use of poly(vinylpyrrolidone) and poly(vinyl alcohol) for cryoultramicrotomy.
1989,
Pubmed Trimmer,
Primary structure and functional expression of a mammalian skeletal muscle sodium channel.
1989,
Pubmed
,
Xenbase Vallon,
Role of Na(+)/H(+) exchanger NHE3 in nephron function: micropuncture studies with S3226, an inhibitor of NHE3.
2000,
Pubmed Waldegger,
Cloning and characterization of SLC26A6, a novel member of the solute carrier 26 gene family.
2001,
Pubmed
,
Xenbase Wang,
Effects of formate and oxalate on volume absorption in rat proximal tubule.
1992,
Pubmed Wang,
Mechanism of proximal tubule bicarbonate absorption in NHE3 null mice.
1999,
Pubmed Wareing,
Effect of formate and oxalate on fluid reabsorption from the proximal convoluted tubule of the anaesthetized rat.
1994,
Pubmed Wu,
Role of NHE3 in mediating renal brush border Na+-H+ exchange. Adaptation to metabolic acidosis.
1996,
Pubmed Zheng,
Prestin is the motor protein of cochlear outer hair cells.
2000,
Pubmed
