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Transforming growth factor beta (TGFbeta) signaling is transduced via Smad2-Smad4-DNA-binding protein complexes which bind to responsive elements in the promoters of target genes. However, the mechanism of how the complexes activate the target genes is unclear. Here we identify Xenopus Swift, a novel nuclear BRCT (BRCA1 C-terminal) domain protein that physically interacts with Smad2 via its BRCT domains. We examine the activity of Swift in relation to gene activation in Xenopus embryos. Swift mRNA has an expression pattern similar to that of Smad2. Swift has intrinsic transactivation activity and activates target gene transcription in a TGFbeta-Smad2-dependent manner. Inhibition of Swift activity results in the suppression of TGFbeta-induced gene transcription and defective mesendoderm development. Blocking Swift function affects neither bone morphogenic protein nor fibroblast growth factor signaling during early development. We conclude that Swift is a novel coactivator of Smad2 and that Swift has a critical role in embryonic TGFbeta-induced gene transcription. Our results suggest that Swift may be a general component of TGFbeta signaling.
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991, Pubmed,
Xenbase
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase Armes,
The ALK-2 and ALK-4 activin receptors transduce distinct mesoderm-inducing signals during early Xenopus development but do not co-operate to establish thresholds.
1997,
Pubmed
,
Xenbase Callebaut,
From BRCA1 to RAP1: a widespread BRCT module closely associated with DNA repair.
1997,
Pubmed Chen,
Smad4 and FAST-1 in the assembly of activin-responsive factor.
1997,
Pubmed
,
Xenbase Chen,
A transcriptional partner for MAD proteins in TGF-beta signalling.
1996,
Pubmed
,
Xenbase Conlon,
Inhibition of Xbra transcription activation causes defects in mesodermal patterning and reveals autoregulation of Xbra in dorsal mesoderm.
1996,
Pubmed
,
Xenbase Ecochard,
A novel Xenopus mix-like gene milk involved in the control of the endomesodermal fates.
1998,
Pubmed
,
Xenbase Escher,
Conservation of glutamine-rich transactivation function between yeast and humans.
2000,
Pubmed Faure,
Endogenous patterns of TGFbeta superfamily signaling during early Xenopus development.
2000,
Pubmed
,
Xenbase Feng,
The tumor suppressor Smad4/DPC4 and transcriptional adaptor CBP/p300 are coactivators for smad3 in TGF-beta-induced transcriptional activation.
1998,
Pubmed Frisch,
XBMPRII, a novel Xenopus type II receptor mediating BMP signaling in embryonic tissues.
1998,
Pubmed
,
Xenbase Fuks,
DNA methyltransferase Dnmt1 associates with histone deacetylase activity.
2000,
Pubmed Germain,
Homeodomain and winged-helix transcription factors recruit activated Smads to distinct promoter elements via a common Smad interaction motif.
2000,
Pubmed
,
Xenbase Graff,
Xenopus Mad proteins transduce distinct subsets of signals for the TGF beta superfamily.
1996,
Pubmed
,
Xenbase Green,
Graded changes in dose of a Xenopus activin A homologue elicit stepwise transitions in embryonic cell fate.
1990,
Pubmed
,
Xenbase Green,
Responses of embryonic Xenopus cells to activin and FGF are separated by multiple dose thresholds and correspond to distinct axes of the mesoderm.
1992,
Pubmed
,
Xenbase Gurdon,
Activin signalling and response to a morphogen gradient.
1994,
Pubmed
,
Xenbase Hemmati-Brivanlou,
A truncated activin receptor inhibits mesoderm induction and formation of axial structures in Xenopus embryos.
1992,
Pubmed
,
Xenbase Henry,
Mixer, a homeobox gene required for endoderm development.
1998,
Pubmed
,
Xenbase Hoodless,
Dominant-negative Smad2 mutants inhibit activin/Vg1 signaling and disrupt axis formation in Xenopus.
1999,
Pubmed
,
Xenbase Janknecht,
TGF-beta-stimulated cooperation of smad proteins with the coactivators CBP/p300.
1998,
Pubmed Jaynes,
Active repression of transcription by the engrailed homeodomain protein.
1991,
Pubmed Kato,
Neuralization of the Xenopus embryo by inhibition of p300/ CREB-binding protein function.
1999,
Pubmed
,
Xenbase Koonin,
BRCA1 protein products ... Functional motifs..
1996,
Pubmed Labbé,
Smad2 and Smad3 positively and negatively regulate TGF beta-dependent transcription through the forkhead DNA-binding protein FAST2.
1998,
Pubmed
,
Xenbase LaBonne,
Mesoderm induction by activin requires FGF-mediated intracellular signals.
1994,
Pubmed
,
Xenbase Lechner,
PTIP, a novel BRCT domain-containing protein interacts with Pax2 and is associated with active chromatin.
2000,
Pubmed Margolis,
cDNAs with long CAG trinucleotide repeats from human brain.
1997,
Pubmed Massagué,
Transcriptional control by the TGF-beta/Smad signaling system.
2000,
Pubmed Massagué,
TGF-beta signal transduction.
1998,
Pubmed McDowell,
Activin has direct long-range signalling activity and can form a concentration gradient by diffusion.
1997,
Pubmed
,
Xenbase Pouponnot,
Physical and functional interaction of SMADs and p300/CBP.
1998,
Pubmed Ryan,
Eomesodermin, a key early gene in Xenopus mesoderm differentiation.
1996,
Pubmed
,
Xenbase Sadowski,
GAL4-VP16 is an unusually potent transcriptional activator.
1988,
Pubmed Shiama,
The p300/CBP family: integrating signals with transcription factors and chromatin.
1997,
Pubmed Shimizu,
Cell-free assay system for Ras- and Rap1-dependent activation of MAP-kinase cascade.
1998,
Pubmed
,
Xenbase Shimizu,
A quantitative analysis of signal transduction from activin receptor to nucleus and its relevance to morphogen gradient interpretation.
1999,
Pubmed
,
Xenbase Smith,
Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos.
1992,
Pubmed
,
Xenbase Takaishi,
Regulation of cell-cell adhesion by rac and rho small G proteins in MDCK cells.
1997,
Pubmed Vojtek,
Mammalian Ras interacts directly with the serine/threonine kinase Raf.
1993,
Pubmed Watanabe,
FAST-1 is a key maternal effector of mesoderm inducers in the early Xenopus embryo.
1999,
Pubmed
,
Xenbase Weinstein,
Neural induction in Xenopus laevis: evidence for the default model.
1997,
Pubmed
,
Xenbase Wilson,
Concentration-dependent patterning of the Xenopus ectoderm by BMP4 and its signal transducer Smad1.
1997,
Pubmed
,
Xenbase Zhou,
Characterization of human FAST-1, a TGF beta and activin signal transducer.
1998,
Pubmed
,
Xenbase Zhu,
Smad3 mutant mice develop metastatic colorectal cancer.
1998,
Pubmed
,
Xenbase Zorn,
Regulation of Wnt signaling by Sox proteins: XSox17 alpha/beta and XSox3 physically interact with beta-catenin.
1999,
Pubmed
,
Xenbase
