Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Signals derived from nonhematopoietic tissues are essential for normal primitive erythropoiesis in vertebrates, but little is known about the nature of these signals. In Xenopus, unidentified factors secreted by ectodermal cells during gastrulation are required to enable the underlying ventral mesoderm to form blood. Steel is expressed in the ectoderm of early Xenopus embryos and is known to regulate definitive erythroid progenitor survival and differentiation in other organisms, making it an excellent candidate regulator of primitive erythropoiesis. In this study, we tested whether steel signaling is required for primitive red blood cell differentiation in mice and frogs. We show that Xsl is expressed in the ectoderm in Xenopus gastrulae and that c-kit homologs are expressed in the underlying mesoderm at the same stages of development. We present loss of function data in whole Xenopus embryos and explants that demonstrate a requirement for ectodermally derived steel to signal through c-kit in the mesoderm to support early steps in the differentiation of primitive erythroid but not myeloid cells. Finally, we show that primitive erythropoiesis is not disrupted in mouse embryos that lack c-kit function. Our data suggest a previously unrecognized and unique function of steel/c-kit during primitive erythropoiesis in Xenopus.
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991, Pubmed,
Xenbase
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase Ashman,
The biology of stem cell factor and its receptor C-kit.
1999,
Pubmed Baker,
A Xenopus c-kit-related receptor tyrosine kinase expressed in migrating stem cells of the lateral line system.
1995,
Pubmed
,
Xenbase Baron,
Embryonic origins of mammalian hematopoiesis.
2003,
Pubmed Baron,
Molecular regulation of embryonic hematopoiesis and vascular development: a novel pathway.
2001,
Pubmed Belaoussoff,
Hematopoietic induction and respecification of A-P identity by visceral endoderm signaling in the mouse embryo.
1998,
Pubmed Bertwistle,
GATA factors and the origins of adult and embryonic blood in Xenopus: responses to retinoic acid.
1996,
Pubmed
,
Xenbase Broudy,
Stem cell factor and hematopoiesis.
1997,
Pubmed Christian,
Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus.
1993,
Pubmed
,
Xenbase Ciau-Uitz,
Distinct origins of adult and embryonic blood in Xenopus.
2000,
Pubmed
,
Xenbase Cui,
Xwnt-8b: a maternally expressed Xenopus Wnt gene with a potential role in establishing the dorsoventral axis.
1995,
Pubmed
,
Xenbase Cumano,
Lymphoid potential, probed before circulation in mouse, is restricted to caudal intraembryonic splanchnopleura.
1996,
Pubmed Evans,
Developmental biology of hematopoiesis.
1997,
Pubmed Galloway,
Loss of gata1 but not gata2 converts erythropoiesis to myelopoiesis in zebrafish embryos.
2005,
Pubmed Galloway,
Ontogeny of hematopoiesis: examining the emergence of hematopoietic cells in the vertebrate embryo.
2003,
Pubmed
,
Xenbase Godin,
Para-aortic splanchnopleura from early mouse embryos contains B1a cell progenitors.
1993,
Pubmed Hayashi,
Exon skipping by mutation of an authentic splice site of c-kit gene in W/W mouse.
1991,
Pubmed Huang,
The hematopoietic growth factor KL is encoded by the Sl locus and is the ligand of the c-kit receptor, the gene product of the W locus.
1990,
Pubmed Kao,
Expression of Xkl-1, a Xenopus gene related to mammalian c-kit, in dorsal embryonic tissue.
1995,
Pubmed
,
Xenbase Kau,
Dual contribution of embryonic ventral blood island and dorsal lateral plate mesoderm during ontogeny of hemopoietic cells in Xenopus laevis.
1983,
Pubmed
,
Xenbase Kelley,
Ventral expression of GATA-1 and GATA-2 in the Xenopus embryo defines induction of hematopoietic mesoderm.
1994,
Pubmed
,
Xenbase Keshet,
Embryonic RNA expression patterns of the c-kit receptor and its cognate ligand suggest multiple functional roles in mouse development.
1991,
Pubmed Kikkawa,
Two-step induction of primitive erythrocytes in Xenopus laevis embryos: signals from the vegetal endoderm and the overlying ectoderm.
2001,
Pubmed
,
Xenbase Kingsley,
Yolk sac-derived primitive erythroblasts enucleate during mammalian embryogenesis.
2004,
Pubmed Kumano,
Spatial and temporal properties of ventral blood island induction in Xenopus laevis.
1999,
Pubmed
,
Xenbase Lane,
The origins of primitive blood in Xenopus: implications for axial patterning.
1999,
Pubmed
,
Xenbase Lennartsson,
Normal and oncogenic forms of the receptor tyrosine kinase kit.
2005,
Pubmed Lennon,
The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression.
1996,
Pubmed Lin,
Differential effects of an erythropoietin receptor gene disruption on primitive and definitive erythropoiesis.
1996,
Pubmed Maéno,
Differential participation of ventral and dorsolateral mesoderms in the hemopoiesis of Xenopus, as revealed in diploid-triploid or interspecific chimeras.
1985,
Pubmed
,
Xenbase Maéno,
Regulatory signals and tissue interactions in the early hematopoietic cell differentiation in Xenopus laevis embryo.
2003,
Pubmed
,
Xenbase Maéno,
Regulation of primary erythropoiesis in the ventral mesoderm of Xenopus gastrula embryo: evidence for the expression of a stimulatory factor(s) in animal pole tissue.
1994,
Pubmed
,
Xenbase Maéno,
Positive and Negative Regulation of the Differentiation of Ventral Mesoderm for Erythrocytes in Xenopus laevis: (Xenopus laevis/erythropoiesis/embryonic blood island/explant/regulation).
1992,
Pubmed
,
Xenbase Martin,
The developmental expression of two Xenopus laevis steel homologues, Xsl-1 and Xsl-2.
2004,
Pubmed
,
Xenbase Matsui,
Embryonic expression of a haematopoietic growth factor encoded by the Sl locus and the ligand for c-kit.
1990,
Pubmed McGann,
Erythropoietin-receptor expression and function during the initiation of murine yolk sac erythropoiesis.
1997,
Pubmed Medvinsky,
Definitive hematopoiesis is autonomously initiated by the AGM region.
1996,
Pubmed Medvinsky,
An early pre-liver intraembryonic source of CFU-S in the developing mouse.
1993,
Pubmed Mellgren,
kitb, a second zebrafish ortholog of mouse Kit.
2005,
Pubmed Miura,
Tissue interaction and the formation of the first erythroblasts of the chick embryo.
1969,
Pubmed Nakayama,
Xenopus Smad8 acts downstream of BMP-4 to modulate its activity during vertebrate embryonic patterning.
1998,
Pubmed
,
Xenbase Nocka,
Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W.
1990,
Pubmed Ogawa,
Expression and function of c-Kit in fetal hemopoietic progenitor cells: transition from the early c-Kit-independent to the late c-Kit-dependent wave of hemopoiesis in the murine embryo.
1993,
Pubmed Ong,
Murine stem cell factor stimulates erythropoietic differentiation of ventral mesoderm in Xenopus gastrula embryo.
1993,
Pubmed
,
Xenbase Palacios,
Developmentally regulated cell surface expression and function of c-kit receptor during lymphocyte ontogeny in the embryo and adult mice.
1992,
Pubmed Parichy,
Zebrafish sparse corresponds to an orthologue of c-kit and is required for the morphogenesis of a subpopulation of melanocytes, but is not essential for hematopoiesis or primordial germ cell development.
1999,
Pubmed Parker,
Cell-autonomous and non-autonomous requirements for the zebrafish gene cloche in hematopoiesis.
1999,
Pubmed Peng,
Xenopus laevis: Practical uses in cell and molecular biology. Solutions and protocols.
1991,
Pubmed
,
Xenbase Reedy,
The expression patterns of c-kit and Sl in chicken embryos suggest unexpected roles for these genes in somite and limb development.
2003,
Pubmed Rhodes,
Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish.
2005,
Pubmed Rohde,
A crucial interaction between embryonic red blood cell progenitors and paraxial mesoderm revealed in spadetail embryos.
2004,
Pubmed Rönnstrand,
Signal transduction via the stem cell factor receptor/c-Kit.
2004,
Pubmed Russel,
Analysis of effects of W and f genic substitutions on fetal mouse hematology.
1968,
Pubmed Smith,
XPOX2-peroxidase expression and the XLURP-1 promoter reveal the site of embryonic myeloid cell development in Xenopus.
2002,
Pubmed
,
Xenbase Tracey,
A Xenopus homologue of aml-1 reveals unexpected patterning mechanisms leading to the formation of embryonic blood.
1998,
Pubmed
,
Xenbase Walters,
Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors.
2002,
Pubmed
,
Xenbase Waskow,
Viable c-Kit(W/W) mutants reveal pivotal role for c-kit in the maintenance of lymphopoiesis.
2002,
Pubmed Wayman,
CaM kinase IV regulates lineage commitment and survival of erythroid progenitors in a non-cell-autonomous manner.
2000,
Pubmed
,
Xenbase Zhang,
Differential regulation of the two xGATA-1 genes during Xenopus development.
1994,
Pubmed
,
Xenbase Zon,
Expression of GATA-binding proteins during embryonic development in Xenopus laevis.
1991,
Pubmed
,
Xenbase
