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Eukaryotic replication origins are 'licensed' for replication early in the cell cycle by loading Mcm(2-7) proteins. As chromatin replicates, Mcm(2-7) are removed, thus preventing the origin from firing again. Here we report the purification of the RLF-B component of the licensing system and show that it corresponds to Cdt1. RLF-B/Cdt1 was inhibited by geminin, a protein that is degraded during late mitosis. Immunodepletion of geminin from metaphase extracts allowed them to assemble licensed replication origins. Inhibition of CDKs in metaphase stimulated origin assembly only after the depletion of geminin. These experiments suggest that geminin-mediated inhibition of RLF-B/Cdt1 is essential for repressing origin assembly late in the cell cycle of higher eukaryotes.
Blow,
Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor.
1993, Pubmed,
Xenbase
Blow,
Preventing re-replication of DNA in a single cell cycle: evidence for a replication licensing factor.
1993,
Pubmed
,
Xenbase Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase Broek,
Involvement of p34cdc2 in establishing the dependency of S phase on mitosis.
1991,
Pubmed Chong,
Characterization of the Xenopus replication licensing system.
1997,
Pubmed
,
Xenbase Chong,
A double-hexamer archaeal minichromosome maintenance protein is an ATP-dependent DNA helicase.
2000,
Pubmed Chong,
Purification of an MCM-containing complex as a component of the DNA replication licensing system.
1995,
Pubmed
,
Xenbase Cocker,
An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast.
1996,
Pubmed Coleman,
The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts.
1996,
Pubmed
,
Xenbase Correa-Bordes,
p25rum1 orders S phase and mitosis by acting as an inhibitor of the p34cdc2 mitotic kinase.
1995,
Pubmed Dahmann,
S-phase-promoting cyclin-dependent kinases prevent re-replication by inhibiting the transition of replication origins to a pre-replicative state.
1995,
Pubmed Diffley,
Once and only once upon a time: specifying and regulating origins of DNA replication in eukaryotic cells.
1996,
Pubmed Diffley,
Two steps in the assembly of complexes at yeast replication origins in vivo.
1994,
Pubmed
,
Xenbase Drury,
The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast.
1997,
Pubmed Elsasser,
Phosphorylation controls timing of Cdc6p destruction: A biochemical analysis.
1999,
Pubmed Gillespie,
Nucleoplasmin-mediated chromatin remodelling is required for Xenopus sperm nuclei to become licensed for DNA replication.
2000,
Pubmed
,
Xenbase Hayles,
Temporal order of S phase and mitosis in fission yeast is determined by the state of the p34cdc2-mitotic B cyclin complex.
1994,
Pubmed Hennessy,
Subcellular localization of yeast CDC46 varies with the cell cycle.
1990,
Pubmed Hua,
A role for Cdk2 kinase in negatively regulating DNA replication during S phase of the cell cycle.
1997,
Pubmed
,
Xenbase Hua,
Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2.
1998,
Pubmed
,
Xenbase Ishimi,
A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.
1997,
Pubmed Jiang,
Multistep regulation of DNA replication by Cdk phosphorylation of HsCdc6.
1999,
Pubmed Kelman,
The single minichromosome maintenance protein of Methanobacterium thermoautotrophicum DeltaH contains DNA helicase activity.
1999,
Pubmed Kubota,
Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs.
1997,
Pubmed
,
Xenbase Kubota,
Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts.
1993,
Pubmed
,
Xenbase Kubota,
Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor.
1995,
Pubmed
,
Xenbase Labib,
Uninterrupted MCM2-7 function required for DNA replication fork progression.
2000,
Pubmed Labib,
G1-phase and B-type cyclins exclude the DNA-replication factor Mcm4 from the nucleus.
1999,
Pubmed Luca,
Control of programmed cyclin destruction in a cell-free system.
1989,
Pubmed Madine,
Geminin bans replication licence.
2001,
Pubmed
,
Xenbase Madine,
MCM3 complex required for cell cycle regulation of DNA replication in vertebrate cells.
1995,
Pubmed
,
Xenbase Mahbubani,
Cell cycle regulation of the replication licensing system: involvement of a Cdk-dependent inhibitor.
1997,
Pubmed
,
Xenbase Maiorano,
XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis.
2000,
Pubmed
,
Xenbase McGarry,
Geminin, an inhibitor of DNA replication, is degraded during mitosis.
1998,
Pubmed
,
Xenbase Meijer,
Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5.
1997,
Pubmed
,
Xenbase Nguyen,
Clb/Cdc28 kinases promote nuclear export of the replication initiator proteins Mcm2-7.
2000,
Pubmed Nishitani,
The Cdt1 protein is required to license DNA for replication in fission yeast.
2000,
Pubmed Noton,
CDK inactivation is the only essential function of the APC/C and the mitotic exit network proteins for origin resetting during mitosis.
2000,
Pubmed Petersen,
Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization.
1999,
Pubmed Piatti,
Activation of S-phase-promoting CDKs in late G1 defines a "point of no return" after which Cdc6 synthesis cannot promote DNA replication in yeast.
1996,
Pubmed Prokhorova,
Sequential MCM/P1 subcomplex assembly is required to form a heterohexamer with replication licensing activity.
2000,
Pubmed
,
Xenbase Romanowski,
The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin.
1996,
Pubmed
,
Xenbase Rowles,
Changes in association of the Xenopus origin recognition complex with chromatin on licensing of replication origins.
1999,
Pubmed
,
Xenbase Rowles,
Interaction between the origin recognition complex and the replication licensing system in Xenopus.
1996,
Pubmed
,
Xenbase Saha,
Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase.
1998,
Pubmed Shechter,
The intrinsic DNA helicase activity of Methanobacterium thermoautotrophicum delta H minichromosome maintenance protein.
2000,
Pubmed Sun,
The replication capacity of intact mammalian nuclei in Xenopus egg extracts declines with quiescence, but the residual DNA synthesis is independent of Xenopus MCM proteins.
2000,
Pubmed
,
Xenbase Tada,
The RLF-B component of the replication licensing system is distinct from Cdc6 and functions after Cdc6 binds to chromatin.
1999,
Pubmed
,
Xenbase Tada,
The replication licensing system.
1998,
Pubmed
,
Xenbase Thömmes,
The RLF-M component of the replication licensing system forms complexes containing all six MCM/P1 polypeptides.
1997,
Pubmed
,
Xenbase Veselý,
Inhibition of cyclin-dependent kinases by purine analogues.
1994,
Pubmed
,
Xenbase Whittaker,
Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts.
2000,
Pubmed
