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Popov AV, Pozniakovsky A, Arnal I, Antony C, Ashford AJ, Kinoshita K, Tournebize R, Hyman AA, Karsenti E.
???displayArticle.abstract??? XMAP215 belongs to a family of proteins involved in the regulation of microtubule dynamics. In this study we analyze the function of different parts of XMAP215 in vivo and in Xenopus egg extracts. XMAP215 has been divided into three fragments, FrN, FrM and FrC (for N-terminal, middle and C-terminal, respectively). FrN co-localizes with microtubules in egg extracts but not in cells, FrC co- localizes with microtubules and centrosomes both in egg extracts and in cells, while FrM does not co- localize with either centrosomes or microtubules. In Xenopus egg extracts, FrN stimulates microtubule growth at plus-ends by inhibiting catastrophes, while FrM has no effect, and FrC suppresses microtubule growth by promoting catastrophes. Our results suggest that XMAP215 is targeted to centrosomes and microtubules mainly through its C-terminal domain, while the evolutionarily conserved N-terminal domain contains its microtubule-stabilizing activity.
Andersen,
XMAP310: a Xenopus rescue-promoting factor localized to the mitotic spindle.
1997, Pubmed,
Xenbase
Andersen,
XMAP310: a Xenopus rescue-promoting factor localized to the mitotic spindle.
1997,
Pubmed
,
Xenbase Andersen,
Effect on microtubule dynamics of XMAP230, a microtubule-associated protein present in Xenopus laevis eggs and dividing cells.
1994,
Pubmed
,
Xenbase Belmont,
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.
1996,
Pubmed
,
Xenbase Chang,
Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins reveal new aspects of centrosome structure and function.
2000,
Pubmed Chapin,
Microtubule stabilization by assembly-promoting microtubule-associated proteins: a repeat performance.
1992,
Pubmed Charrasse,
The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215.
1998,
Pubmed
,
Xenbase Cullen,
mini spindles: A gene encoding a conserved microtubule-associated protein required for the integrity of the mitotic spindle in Drosophila.
1999,
Pubmed
,
Xenbase Drewes,
MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption.
1997,
Pubmed Ebneth,
Phosphorylation of MAP2c and MAP4 by MARK kinases leads to the destabilization of microtubules in cells.
1999,
Pubmed Gard,
A microtubule-associated protein from Xenopus eggs that specifically promotes assembly at the plus-end.
1987,
Pubmed
,
Xenbase Gräf,
Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication.
2000,
Pubmed Le Bot,
Role of xklp3, a subunit of the Xenopus kinesin II heterotrimeric complex, in membrane transport between the endoplasmic reticulum and the Golgi apparatus.
1998,
Pubmed
,
Xenbase Matthews,
ZYG-9, a Caenorhabditis elegans protein required for microtubule organization and function, is a component of meiotic and mitotic spindle poles.
1998,
Pubmed McNally,
Katanin, the microtubule-severing ATPase, is concentrated at centrosomes.
1996,
Pubmed Miller,
Comparison of in vivo and in vitro ribosomal RNA synthesis in nucleolar mutants of Xenopus laevis.
1977,
Pubmed
,
Xenbase Moens,
Spindle and kinetochore morphology of Dictyostelium discoideum.
1976,
Pubmed Nabeshima,
p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites.
1995,
Pubmed Nakaseko,
Dissection of fission yeast microtubule associating protein p93Dis1: regions implicated in regulated localization and microtubule interaction.
1996,
Pubmed Sawin,
Mitotic spindle assembly by two different pathways in vitro.
1991,
Pubmed
,
Xenbase Smrzka,
Tissue-specific expression and subcellular localisation of mammalian delta-tubulin.
2000,
Pubmed Spittle,
The interaction of TOGp with microtubules and tubulin.
2000,
Pubmed
,
Xenbase Tournebize,
Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis.
1997,
Pubmed
,
Xenbase Tournebize,
Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase Verde,
Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase Walczak,
XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.
1996,
Pubmed
,
Xenbase Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed Wang,
Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body.
1997,
Pubmed Wiese,
A new function for the gamma-tubulin ring complex as a microtubule minus-end cap.
2000,
Pubmed
,
Xenbase Wilde,
Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran.
1999,
Pubmed
,
Xenbase Wittmann,
Localization of the kinesin-like protein Xklp2 to spindle poles requires a leucine zipper, a microtubule-associated protein, and dynein.
1998,
Pubmed
,
Xenbase
