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Quiescent full-grown Xenopus oocytes remain arrested at the G(2)/M border of meiosis I until exposed to progesterone, their natural mitogen. Progesterone triggers rapid, nontranscriptional responses that lead to the translational activation of stored mRNAs, resumption of the meiotic cell cycles, and maturation of the oocyte into a fertilizable egg. It has long been presumed that progesterone activates the oocyte through a novel nontranscriptional signaling receptor. Here, we provide evidence that a conventional transcriptional progesterone receptor cloned from Xenopus oocytes, XPR-1, is required for oocyte activation. Overexpression of XPR-1 through mRNA injection increases sensitivity to progesterone and accelerates progesterone-activated cell cycle reentry. Injection of XPR-1 antisense oligonucleotides blocks the ability of oocytes to respond to progesterone; these oocytes are rescued by subsequent injection of XPR-1 or the human progesterone receptor PR-B. Antisense-treated oocytes can be activated in response to inhibition of protein kinase A, one of the earliest known changes occurring downstream of progesterone stimulation. These results argue that the conventional progesterone receptor also functions as the signaling receptor that is responsible for the rapid nontranscriptional activation of frog oocytes.
Akamatsu,
Transcription factor E2F and cyclin E-Cdk2 complex cooperate to induce chromosomal DNA replication in Xenopus oocytes.
1998, Pubmed,
Xenbase
Akamatsu,
Transcription factor E2F and cyclin E-Cdk2 complex cooperate to induce chromosomal DNA replication in Xenopus oocytes.
1998,
Pubmed
,
Xenbase Andrésson,
The kinase Eg2 is a component of the Xenopus oocyte progesterone-activated signaling pathway.
1998,
Pubmed
,
Xenbase Bandyopadhyay,
Plasma membrane mediated action of progesterone in amphibian (Rana dybowskii) oocyte maturation.
1998,
Pubmed Castoria,
Non-transcriptional action of oestradiol and progestin triggers DNA synthesis.
1999,
Pubmed Chen,
Estrogen receptor alpha mediates the nongenomic activation of endothelial nitric oxide synthase by estrogen.
1999,
Pubmed Clarke,
Perimembrane localization of the estrogen receptor alpha protein in neuronal processes of cultured hippocampal neurons.
2000,
Pubmed Cork,
Second messenger signalling during hormone-induced Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase Ferrell,
Xenopus oocyte maturation: new lessons from a good egg.
1999,
Pubmed
,
Xenbase Finidori-Lepicard,
Progesterone inhibits membrane-bound adenylate cyclase in Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase Fortune,
Ovarian progesterone levels during in vitro oocyte maturation and ovulation in Xenopus laevis.
1975,
Pubmed
,
Xenbase Godeau,
Induction of maturation in Xenopus laevis oocytes by a steroid linked to a polymer.
1978,
Pubmed
,
Xenbase Horwitz,
In situ photolinked nuclear progesterone receptors of human breast cancer cells: subunit molecular weights after transformation and translocation.
1983,
Pubmed Ishikawa,
Primary action of steroid hormone at the surface of amphibian oocyte in the induction of germinal vesicle breakdown.
1977,
Pubmed
,
Xenbase Iwashita,
Essential role of germinal vesicle material in the meiotic cell cycle of Xenopus oocytes.
1998,
Pubmed
,
Xenbase Kastner,
Two distinct estrogen-regulated promoters generate transcripts encoding the two functionally different human progesterone receptor forms A and B.
1990,
Pubmed Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
,
Xenbase Maller,
The elusive progesterone receptor in Xenopus oocytes.
2001,
Pubmed
,
Xenbase Maller,
Recurring themes in oocyte maturation.
1998,
Pubmed Maller,
Regulation of amphibian oocyte maturation.
1985,
Pubmed
,
Xenbase Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed Masui,
Relative roles of the pituitary, follicle cells, and progesterone in the induction of oocyte maturation in Rana pipiens.
1967,
Pubmed McEwan,
Gene regulation through chromatin remodelling by members of the nuclear receptor superfamily.
2000,
Pubmed McKenna,
Nuclear receptor coactivators: multiple enzymes, multiple complexes, multiple functions.
1999,
Pubmed Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase Migliaccio,
Activation of the Src/p21ras/Erk pathway by progesterone receptor via cross-talk with estrogen receptor.
1998,
Pubmed Migliaccio,
Immediate and transient stimulation of protein tyrosine phosphorylation by estradiol in MCF-7 cells.
1993,
Pubmed Migliaccio,
Tyrosine kinase/p21ras/MAP-kinase pathway activation by estradiol-receptor complex in MCF-7 cells.
1996,
Pubmed Morrill,
Progesterone induces meiotic division in the amphibian oocyte by releasing lipid second messengers from the plasma membrane.
1999,
Pubmed Morrill,
Progesterone binding to plasma membrane and cytosol receptors in the amphibian oocyte.
1997,
Pubmed Morrison,
Nongenomic action of progesterone: activation of Xenopus oocyte phospholipase C through a plasma membrane-associated tyrosine kinase.
2000,
Pubmed
,
Xenbase Murakami,
Mechanisms of Xenopus oocyte maturation.
1997,
Pubmed
,
Xenbase Nagahama,
17 alpha,20 beta-dihydroxy-4-pregnen-3-one, a maturation-inducing hormone in fish oocytes: mechanisms of synthesis and action.
1997,
Pubmed Palmer,
The activation of MAP kinase and p34cdc2/cyclin B during the meiotic maturation of Xenopus oocytes.
2000,
Pubmed
,
Xenbase Razandi,
Cell membrane and nuclear estrogen receptors (ERs) originate from a single transcript: studies of ERalpha and ERbeta expressed in Chinese hamster ovary cells.
1999,
Pubmed Revelli,
Nongenomic actions of steroid hormones in reproductive tissues.
1998,
Pubmed Russell,
Human vascular endothelial cells contain membrane binding sites for estradiol, which mediate rapid intracellular signaling.
2000,
Pubmed Sadler,
Progesterone inhibits adenylate cyclase in Xenopus oocytes. Action on the guanine nucleotide regulatory protein.
1981,
Pubmed
,
Xenbase Sagata,
What does Mos do in oocytes and somatic cells?
1997,
Pubmed
,
Xenbase Schuetz,
Action of hormones on germinal vesicle breakdown in frog (Rana pipiens) oocytes.
1967,
Pubmed Smith,
Role of the oocyte nucleus in physiological maturation in Rana pipiens.
1969,
Pubmed Smith,
The interaction of steroids with Rana pipiens Oocytes in the induction of maturation.
1971,
Pubmed Smith,
In vitro induction of physiological maturation in Rana pipiens oocytes removed from their ovarian follicles.
1968,
Pubmed Takimoto,
Role of phosphorylation on DNA binding and transcriptional functions of human progesterone receptors.
1996,
Pubmed Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase Wasserman,
A study on gonadotropin action in the induction of oocyte maturation in Xenopus laevis.
1974,
Pubmed
,
Xenbase Weatherman,
Nuclear-receptor ligands and ligand-binding domains.
1999,
Pubmed Wehling,
Specific, nongenomic actions of steroid hormones.
1997,
Pubmed Xu,
Coactivator and corepressor complexes in nuclear receptor function.
1999,
Pubmed Yamashita,
Molecular mechanisms of the initiation of oocyte maturation: general and species-specific aspects.
2000,
Pubmed
