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Summary Attributions Wiki
XB-GRNA-23659080
gRNA Name: lhx1 gRNA1
Synonyms:
Genomic Target: lhx1

Sequence: 5' GGAGAAATGCTTCTCCAGGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/20 19/20 19/20
genome
gRNA
 
5' CCCTGGAGAAGCATTTCTCG 3'
   ||||||||||||||||||| 
3' GGGACCTCTTCGTAAAGAGG 5'
 
5' CCCTGGAGAAGCATTTCTCG 3'
   ||||||||||||||||||| 
3' GGGACCTCTTCGTAAAGAGG 5'
 
5' CCCTGGAGAAGCATTTCTCG 3'
   ||||||||||||||||||| 
3' GGGACCTCTTCGTAAAGAGG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2L:63770764..63770783 19/20 Sense target lhx1.L
Chr2S:65455574..65455593 19/20 Sense target lhx1.S
Chr2L:160313393..160313412 16/20 Sense off-target postn.L
Chr2S:143329791..143329810 16/20 Sense off-target postn.S
Chr5L:19528884..19528903 19/20 unknown
Chr7S:6987829..6987848 18/20 unknown
Chr3L:85128097..85128116 17/20 unknown
Chr4L:88452531..88452550 16/20 unknown
Chr9_10L:58160977..58160996 16/20 unknown
tropicalis 10.0 Chr2:75675485..75675504 19/20 Sense target lhx1
Chr1:146145738..146145757 18/20 Sense off-target lhx5
Chr8:125706597..125706616 18/20 Antisense off-target cadm3
Chr5:94409537..94409556 17/20 Sense off-target adgrb3
Chr8:142290862..142290881 16/20 Sense off-target adar
Chr8:125066760..125066779 16/20 unknown
Publications
???morpholino.displaySummary.papersFirst??? Tissue-Specific Gene Inactivation in Xenopus laevis: Knockout of lhx1 in the Kidney with CRISPR/Cas9., Genetics 2018     
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