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Summary Attributions Wiki
XB-GRNA-23659126
gRNA Name: kdm6a gRNA1
Synonyms:
Genomic Target: kdm6a

PAM Sequence: AGG
Sequence: 5' GGTAAAGGTGGATAGGGTGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 20/20 19/20
genome
gRNA
 
5' CCACCCTATCCACCTTTACC 3'
   ||||||||||||||||||||
3' GGTGGGATAGGTGGAAATGG 5'
 
5' CCACCCTATCCACCTTTACC 3'
   ||||||||||||||||||||
3' GGTGGGATAGGTGGAAATGG 5'
 
5' CCACCTTATCCACCTTTACC 3'
   ||||| ||||||||||||||
3' GGTGGGATAGGTGGAAATGG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2L:83270289..83270308 20/20 Antisense target kdm6a.L
Chr2S:47844952..47844971 19/20 Antisense target kdm6a.S
Chr7L:38798318..38798337 18/20 Antisense off-target ascc1.L
Chr1S:130666280..130666299 18/20 Sense off-target cdh24.S
Chr9_10L:24246188..24246207 17/20 Antisense off-target notum.L
Chr3S:114092720..114092739 17/20 Antisense off-target slit3.S
Chr7L:50973674..50973693 16/20 Sense off-target nrg3.L
Chr8L:126073569..126073588 16/20 Antisense off-target btn2a1.L
Chr8L:126073569..126073588 16/20 Sense off-target LOC121397210
Chr6S:47533279..47533298 17/20 unknown
Chr8L:77229237..77229256 16/20 unknown
Chr1S:83147686..83147705 16/20 unknown
Chr2L:188489044..188489063 16/20 unknown
Chr2S:52743713..52743732 16/20 unknown
Chr3S:11142279..11142298 16/20 unknown
Chr5L:64800887..64800906 16/20 unknown
Chr5S:71055544..71055563 16/20 unknown
Chr6S:53482005..53482024 16/20 unknown
Chr6S:55029924..55029943 16/20 unknown
tropicalis 10.0 Chr2:55776368..55776387 20/20 Antisense target kdm6a
Chr8:34306789..34306808 17/20 Antisense off-target pappa
Chr6:99186322..99186341 17/20 Antisense off-target znf516
Chr8:34306789..34306808 17/20 Sense off-target LOC116406671
Chr10:21547192..21547211 16/20 Antisense off-target aoc3
Chr7:64180942..64180961 16/20 Antisense off-target pex14
Chr9:44800744..44800763 16/20 Antisense off-target pgap1
Chr6:73342466..73342485 16/20 Sense off-target tmem245
Chr1:115270873..115270892 18/20 unknown
Chr5:68117036..68117055 18/20 unknown
Chr2:75226187..75226206 16/20 unknown
Chr4:125037846..125037865 16/20 unknown
Chr7:32311710..32311729 16/20 unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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