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Summary Attributions Wiki
XB-GRNA-23659130
gRNA Name: nf1 gRNA2
Synonyms: nf1_g2
Genomic Target: nf1

PAM Sequence: CGG
Sequence: 5' CGAAGCAGACATTCGGAGTT 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 20/20 20/20
genome
gRNA
 
5' AACTCCGAATGTCTGCTTCG 3'
   ||||||||||||||||||||
3' TTGAGGCTTACAGACGAAGC 5'
 
5' AACTCCGAATGTCTGCTTCG 3'
   ||||||||||||||||||||
3' TTGAGGCTTACAGACGAAGC 5'
 
5' AACTCCGAATGTCTGCTTCG 3'
   ||||||||||||||||||||
3' TTGAGGCTTACAGACGAAGC 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2L:107904586..107904605 20/20 Antisense target nf1.L
Chr2S:96171478..96171497 20/20 Antisense target nf1.S
Chr8S:13540446..13540465 18/20 Sense off-target gmfb.S
Chr4S:43127419..43127438 17/20 Antisense off-target LOC108715428
Chr1L:36198771..36198790 19/20 unknown
Chr7L:4022767..4022786 17/20 unknown
tropicalis 10.0 Chr2:104771147..104771166 20/20 Antisense target nf1
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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