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Summary Attributions Wiki
XB-GRNA-23659141
gRNA Name: nog gRNA1
Synonyms:
Genomic Target: nog

PAM Sequence: AGG
Sequence: 5' GGAGGAGAGACTTGGAGTGG 3'



Genomic Target tropicalis laevis.L
Identities 20/20 19/20
genome
gRNA
 
5' CCACTCCAAGTCTCTCCTCC 3'
   ||||||||||||||||||||
3' GGTGAGGTTCAGAGAGGAGG 5'
 
5' CCACTCCAAGTCTCTCCTCT 3'
   ||||||||||||||||||| 
3' GGTGAGGTTCAGAGAGGAGG 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr9_10L:32768149..32768168 19/20 Sense target nog.L
Chr9_10L:113660743..113660762 17/20 Sense off-target LOC121398039
Chr1S:116401333..116401352 17/20 unknown
Chr9_10L:53002502..53002521 16/20 unknown
Chr9_10S:50349910..50349929 16/20 unknown
Chr4S:18171566..18171585 16/20 unknown
tropicalis 10.0 Chr10:25898904..25898923 20/20 Sense target nog
Chr2:147329874..147329893 18/20 Sense off-target rarg
Chr5:23873284..23873303 17/20 Antisense off-target cimip6
Chr8:147092862..147092881 17/20 unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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