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Summary Attributions Wiki
XB-GRNA-23659156
gRNA Name: prickle1 gRNA1
Synonyms: prickle_L
Genomic Target: prickle1

PAM Sequence: AGG
Sequence: 5' TGCGGAGAGAAGATTAATGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 20/20 20/20
genome
gRNA
 
5' CCATTAATCTTCTCTCCGCA 3'
   ||||||||||||||||||||
3' GGTAATTAGAAGAGAGGCGT 5'
 
5' CCATTAATCTTCTCTCCGCA 3'
   ||||||||||||||||||||
3' GGTAATTAGAAGAGAGGCGT 5'
 
5' CCATTAATCTTCTCTCCGCA 3'
   ||||||||||||||||||||
3' GGTAATTAGAAGAGAGGCGT 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr3L:71427092..71427111 20/20 Sense target prickle1.L
Chr3S:83509799..83509818 20/20 Sense target prickle1.S
Chr5L:17313930..17313949 18/20 Antisense off-target wdr26.L
Chr1S:79523808..79523827 18/20 Sense off-target adgrl3.S
Chr1L:182066764..182066783 18/20 Sense off-target slc12a2.L
Chr5S:12645866..12645885 17/20 Antisense off-target wdr26.S
Chr8S:41437054..41437073 16/20 Sense off-target psmb7.S
Chr3L:122893409..122893428 19/20 unknown
Chr3S:71180321..71180340 17/20 unknown
tropicalis 10.0 Chr3:64772903..64772922 20/20 Sense target prickle1
Chr4:119570355..119570374 18/20 Antisense off-target pla2g4a
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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