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Summary Attributions Wiki
XB-GRNA-23659158
gRNA Name: eya1 gRNA1
Synonyms: eya1_L
Genomic Target: eya1

PAM Sequence: AGG
Sequence: 5' GGACATTTAGACAACTTCTC 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 20/20 20/20
genome
gRNA
 
5' GAGAAGTTGTCTAAATGTCC 3'
   ||||||||||||||||||||
3' CTCTTCAACAGATTTACAGG 5'
 
5' GAGAAGTTGTCTAAATGTCC 3'
   ||||||||||||||||||||
3' CTCTTCAACAGATTTACAGG 5'
 
5' GAGAAGTTGTCTAAATGTCC 3'
   ||||||||||||||||||||
3' CTCTTCAACAGATTTACAGG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr6L:129136999..129137018 20/20 Sense target eya1.L
Chr6S:106178513..106178532 20/20 Sense target eya1.S
Chr8L:60861421..60861440 17/20 Antisense off-target map7d3.L
Chr7L:134740244..134740263 16/20 Sense off-target LOC108696888
Chr8L:249231..249250 18/20 unknown
Chr8L:60446614..60446633 18/20 unknown
Chr2S:95861505..95861524 18/20 unknown
Chr7S:44337707..44337726 18/20 unknown
Chr8L:62263826..62263845 17/20 unknown
Chr6L:114614310..114614329 17/20 unknown
Chr2L:21734386..21734405 16/20 unknown
tropicalis 10.0 Chr6:120536282..120536301 20/20 Sense target eya1
Chr8:70249177..70249196 17/20 Antisense off-target fkrp
Chr7:79169046..79169065 17/20 Antisense off-target trim29
Chr7:86494560..86494579 16/20 Antisense off-target ttc12
Chr5:87299564..87299583 16/20 Antisense off-target syncrip
Chr8:72619163..72619182 18/20 unknown
Chr6:58205635..58205654 16/20 unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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