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Summary Attributions Wiki
XB-GRNA-23659712
gRNA Name: pdx1 gRNA2
Synonyms: pdx1-T2
Genomic Target: pdx1

PAM Sequence: tgg
Sequence: 5' GGTGAGGGATCCCTGGGTCA 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 19/20 18/20 18/20
genome
gRNA
 
5' TCACCCAGGGATCCCTCACC 3'
   | ||||||||||||||||||
3' ACTGGGTCCCTAGGGAGTGG 5'
 
5' TCACCCAGGGATCCCTCATC 3'
   | |||||||||||||||| |
3' ACTGGGTCCCTAGGGAGTGG 5'
 
5' TCACCCAGGGATCCCTCATC 3'
   | |||||||||||||||| |
3' ACTGGGTCCCTAGGGAGTGG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2S:147318170..147318189 18/20 Antisense target pdx1.S
Chr2L:164681275..164681294 18/20 Antisense target pdx1.L
Chr1L:38123697..38123716 17/20 Antisense off-target limch1.L
Chr2S:126846469..126846488 16/20 Antisense off-target nr4a1.S
Chr2L:141842525..141842544 16/20 Antisense off-target nr4a1.L
Chr9_10L:10875287..10875306 18/20 unknown
Chr8S:64940052..64940071 18/20 unknown
Chr1L:9486660..9486679 17/20 unknown
Chr1L:9511653..9511672 17/20 unknown
Chr2L:103025396..103025415 17/20 unknown
tropicalis 10.0 Chr2:155160032..155160051 19/20 Antisense target pdx1
Chr7:81210904..81210923 17/20 Antisense off-target kmt2a
Chr8:109991167..109991186 17/20 Sense off-target chrm5
Chr2:135285457..135285476 16/20 Antisense off-target nr4a1
Chr2:86587245..86587264 16/20 Antisense off-target kpna6
Chr2:99223262..99223281 17/20 unknown
Publications
???morpholino.displaySummary.papersFirst??? Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis., Development 2014     
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