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Summary Attributions Wiki
XB-GRNA-25874504

gRNA Name: rho gRNA2
Synonyms: rhosg4
Genomic Target: rho

Sequence: 5' GGGTGGTGATCAAGCAGTTA 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 20/20 20/20 20/20
genome
gRNA
5' TAACTGCTTGATCACCACCC 3'
   ||||||||||||||||||||
3' ATTGACGAACTAGTGGTGGG 5'
5' TAACTGCTTGATCACCACCC 3'
   ||||||||||||||||||||
3' ATTGACGAACTAGTGGTGGG 5'
5' TAACTGCTTGATCACCACCC 3'
   ||||||||||||||||||||
3' ATTGACGAACTAGTGGTGGG 5'
   
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr4S:110977920..110977939 20/20 Antisense target rho.S
Chr4L:131408126..131408145 20/20 Antisense target rho.L
Chr4L:131395781..131395800 20/20 Antisense off-target rho.2.L
Chr5S:100577384..100577403 18/20 Antisense off-target bche.S
Chr1L:152153122..152153141 16/20 Sense off-target LOC101027262
Chr1L:152153122..152153141 16/20 Sense off-target LOC108714884
Chr1L:57498996..57499015 18/20 unknown
Chr7S:81273946..81273965 17/20 unknown
tropicalis 10.0 Chr4:131926665..131926684 20/20 Antisense target rho
Chr2:45190338..45190357 16/20 Sense off-target adgrg2
Chr7:59306211..59306230 16/20 Sense off-target LOC100486241
Chr7:115892551..115892570 19/20 unknown
Chr2:179739929..179739948 17/20 unknown
Publications
???morpholino.displaySummary.papersFirst??? Modeling Dominant and Recessive Forms of Retinitis Pigmentosa by Editing Three Rhodopsin-Encoding Genes in Xenopus Laevis Using Crispr/Cas9., Sci Rep 2017     
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