XB-LINE-1234
Line Name: Xtr.hps6mGrngr
| Summary |
|
| Synonym: |
No Privacy, NOP, noprivacy, T344-C, nop, Xtr.nopmGrngr |
| Species: |
Xenopus tropicalis |
| Background Strain: |
Xtr.Nigerian1EXRC
|
| Description: |
No Privacy (NOP) embryos have a 10-bp deletion in the hps6 gene (a region from 468 to 477 counted from the beginning of the ORF). Mutation identified in the Grainger screen for ENU-induced mutations by gynogenesis. Positional cloning identified the site of mutation in the hps6 gene, which was confirmed by BAC rescue and targeted mutagenesis (by TALEN) of the hps6 gene. Model for Hermansky-Pudlak syndrome. Homozygous animals are viable, transparent. This colony was shared with the EXRC, the NXR, and the Khokha lab. |
| Phenotype Description: |
Markedly reduced pigmentation in the retina and the trunk from NF stage 39 onwards. Reduced production of pigmented melanophores by NF stage 50 and no production of pigmented iridophores. Albinos through to froglet stage, slight pigmentation as adults. Homozygous nop female crossed with with heterozygous males have abnormal inner ear morphology ( enlarged or absent inner ear otoliths, or absent otoliths and enlarged otic vesicle) . |
| Color Morph: |
pigmented |
| Breeding Type: |
INBRED
|
| Lab of Origin: |
Grainger Lab |
| Line Type: |
Mutant |
| Mutated Gene(s): |
hps6
|
| MTA Required: |
No
|
| Public: |
Yes
|
| Anatomical Phenotypes: |
hps6 |
| Disease Phenotypes: |
hps6 |
|
Fig. 1. A single founder mutant embryo was raised to adulthood. (A) The founder mutant was photographed just as it was about to complete metamorphosis, which at this stage is indistinguishable from an albino froglet. (B) The founder mutant as an adult (a wildtype adult is shown in B for comparison). The white dotted square region is enlarged in the black dotted square (B) where one can recognize that the formation of melanin is partially restored. (C) Eggs, which are less pigmented than wildtype (C), were obtained from the adult in (B).
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Fig. 6. Maternal effect of nop mutation leads to otolith defect. Dorsal views, at stage 46, of nop (A) and wildtype (B) embryos derived from the heterozygous mutant parents and nop (C) and wildtype (D) embryos derived from the same heterozygous male as used for (A) and (B) and homozygous female. Only when the nop mutant is derived from a homozygous female are otolith defects seen (white arrows in white-dotted circles) as compared to normal otoliths (black arrows in black-dotted circles). Variation of otolith defects (indicated by white arrows) are seen in nop (EH) compared to the wildtype (I). (Br) Brain, (u) Utricle, (s) Saccule, (Ov) Otic vesicle.
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Fig. 3. Appearance of visceral organs of no privacy and wildtype froglets. (A, B) Ventral view of outer surface of the peritoneum. (C, D) Ventral view of the liver (l), stomach (s), and intestine (i). (E, F) Dorsal view of the kidneys (k) and lungs (lu). (A, C, E) no privacy, (B, D, F) wildtype.
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| Stock Center |
RRID |
Generation |
DOB |
# Males |
# Females |
Availability |
Mutant Details |
| EXRC (Europe) |
EXRC_6003 |
|
| NXR (US) |
NXR_10019 |
|
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