XB-LINE-1531
Line Name: Xtr.smad4mZimml
| Summary |
|
| Synonym: |
white hart , whamh107, T385-C, Xtr.smad4.1mZimml |
| Species: |
Xenopus tropicalis |
| Background Strain: |
Xtr.inbred
|
| Description: |
Mutation affecting haematopoiesis identified in a screen for ENU-induced mutations at Mill Hill. Positional cloning identified it as a nonsense mutation in smad4.1. |
| Phenotype Description: |
Homozygous mutants have little or no circulating blood cells. An unusual lump around the proctodeum is visible as early as ~stage 37, with subsequent ventral fin defects. |
| Color Morph: |
pigmented |
| Breeding Type: |
OUTBRED
|
| Lab of Origin: |
Zimmerman Lab |
| Line Type: |
Mutant |
| Mutated Gene(s): |
smad4
|
| MTA Required: |
Yes
|
| Public: |
Yes
|
| Anatomical Phenotypes: |
smad4 |
|
WISH with α-globin suggests that the blood distribution in mutant Xtr.smad4.1 tadpoles (on right, also known as whitehart or wha) is aberrant, with reduced globin staining pooled ventrally (black arrows) rather than distributed throughout the circulatory system as in wild-type tadpoles (left).
|
|
hba1 (hemoglobin subunit alpha 1) gene expression in Xtr.smad4.1 mutant at tadpole stage, ( also known as whitehart or wha), asayyed bu WISH, ventral view, anterior left. This assays confirms phenotype as defects in blood formation with normal circulation.
|
|
Xtr.smad4.1 mutant embryos. The white hart/wha phenotype includes no circulating blood cells, normal heartbeat, defect in blood formation, the dorsal fin appears 'frilly'.
|
| Stock Center |
RRID |
Generation |
DOB |
# Males |
# Females |
Availability |
Mutant Details |
| EXRC (Europe) |
EXRC_6002 |
|
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