XB-LINE-1536
Line Name: Xtr.supt5hmZimml
| Summary |
|
| Synonym: |
cyd vicious, cyd mh139, T349-C |
| Species: |
Xenopus tropicalis |
| Background Strain: |
Xtr.Nigerian1EXRC
|
| Description: |
Mutation affecting eye development and melanophore migration identified in the Mill Hill screen for ENU-induced mutations. |
| Phenotype Description: |
Pleiotropic effect on eye (retinal lamination defect, no lens), neural crest (poor/absent melanophore migration). Pericardial edema and bent body axis are also observed. Development arrests at ~ NF stage 28-32. |
| Color Morph: |
pigmented |
| Breeding Type: |
OUTBRED
|
| Lab of Origin: |
Zimmerman Lab |
| Line Type: |
Mutant |
| Mutated Gene(s): |
supt5h
|
| MTA Required: |
Yes
|
| Public: |
Yes
|
|
Figure 6. The Mutation cyd vicious [Xtra.supt5h] Displays Neural Crest and Eye Defects
Brightfield images of stage ~38 outcrossed sibling wild-type embryo (A) and gynogenetic cyd embryo (B). Likely neural crest-derived pigmented cells (arrows, [C] and [D]) fail to migrate in cyd, and instead populate the lumen of the neural tube. St. 40 wild-type eye (E) displays laminar organization surrounded by prominent pigmented epithelium. cyd eyes form a poorly laminated ball of neural retina surrounding a central mass of pigmented tissue, and no lens tissue is visible (F).
|
|
Xtr.supt5h mutant embryos (also known as cyd vicious /cyd), melanocytes fail to migrate out of the dorsal neural tube, resulting in pigmented 'mohawk' effect. Pericardial edema and bent body axis are also observed. Development arrests at stage ~28-32.
|
| Stock Center |
RRID |
Generation |
DOB |
# Males |
# Females |
Availability |
Mutant Details |
| EXRC (Europe) |
EXRC_6004 |
|
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