XB-LINE-803
Line Name: Xla.Tg(Xtr.ins:dnthra-eGFP)Brown
| Summary |
|
| Synonym: |
pcs2::Insulin::TRDN-GFP3 |
| Species: |
Xenopus laevis |
| Background Strain: |
Xla.Inbred
|
| Description: |
5.4 kb X. tropicalis insulin promoter driving dominant negative form of the thyroid receptor alpha ( (aka TRDN)) fused to GFP. The pInsulin-TRDN-GFPconstruct was made by amplifying the 5.4 kb fragment of the promoter by PCR, using primers that incorporated a Xho1 site at the 5' end and Sma1 at the 3' end, and this PCR-product was ligated into the pCS2-CMV-TRDN-flex-GFP that had been cleaved by Sal1 (at 5'and HindIII (at 3') replacing the existing CMV promoter sequence. |
| Phenotype Description: |
GFP in pancreatic Beta-cells. |
| Breeding Type: |
UNKNOWN
|
| Lab of Origin: |
Brown Lab |
| Line Type: |
Transgenic |
| Mutated Gene(s): |
|
| MTA Required: |
Yes
|
| Public: |
Yes
|
| Stock Center |
RRID |
Generation |
DOB |
# Males |
# Females |
Availability |
Mutant Details |
|
No availability, contact the Lab of Origin for information about this Xenopus line or strain. Articles using these frogs are listed on the Attribution Tab.
|
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