XB-LINE-920
Line Name: Xla.Tg(kdr:eGFP)Pec
| Summary |
|
| Synonym: |
Xl.Tg(flk1:eGFP) |
| Species: |
Xenopus laevis |
| Background Strain: |
Xla.Inbred
|
| Description: |
The Xenopus laevis Flk1 (VEGFR-2) promoter and first intron were used to drive GFP.
See Figure 1 and Figure 2 in Ny et. Al. Biol Open. 2013 Jul 11; 2(9):882-90.
A genomic fragment containing 2.5 kb of promoter sequence up to exon 2, including the complete intron 1 containing enhancer elements are essential for the correct expression pattern, was isolated and cloned into pBSK(+) Next, the eGFP cDNA fused to the SV40 polyA was inserted into exon 1 to drive reporter expression under the control of the xlFlk1 promoter. REMI method. |
| Phenotype Description: |
GFP expression in blood and lymphatic vessels. Earliest specific GFP signal visible is at NF stage 35-36 (in posterior cardinal vein and intersomitic vessels). GFP signal in all blood vessels and lymphatic vessels from NF stage 42 onwards. |
| Color Morph: |
albino |
| Breeding Type: |
UNKNOWN
|
| Lab of Origin: |
Carmeliet Lab |
| Line Type: |
Transgenic |
| Mutated Gene(s): |
|
| MTA Required: |
No
|
| Public: |
Yes
|
| Catalog Number: |
waiting for EXRC RRID |
| Stock Center |
RRID |
Generation |
DOB |
# Males |
# Females |
Availability |
Mutant Details |
|
No availability, contact the Lab of Origin for information about this Xenopus line or strain. Articles using these frogs are listed on the Attribution Tab.
|
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