XB-LINE-955
Line Name: Xla.Tg(rho:Eco.nfsA)Zuber
| Summary |
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| Synonym: |
XOPNTR; XOP:NTR |
| Species: |
Xenopus laevis |
| Background Strain: |
Xla.Inbred
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| Description: |
rhodopsin (rho) promoter driving E. coli nitroreductase.Plasmid used for transgenesis (pXOP(-508/+41)-NTR) consists of the E. coli nitroreductase (NTR) expressed under the control of the Xenopus laevis rhodopsin promoter from Barry Knox. Base vector is pEGFP-N1 (Clontech) lacking CMV immediate early promoter & eGFP. |
| Phenotype Description: |
Expression pattern is best detected by in situ hybridization for nitroreductase transgene and is expressed specifically in rod photoreceptors
Transgenic animals express nitroreductase in rod photoreceptors. Expression of the transgene has no deleterious effect on the development or survival of transgenic animals. However, addition of Metronidazole results in rapid, specific death of rod cells
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| Breeding Type: |
UNKNOWN
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| Lab of Origin: |
Zuber Lab |
| Line Type: |
Transgenic |
| Mutated Gene(s): |
|
| MTA Required: |
No
|
| Public: |
Yes
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| Catalog Number: |
EXRC_0049 |
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Figure 2 . Lateral view of a transgenic tadpole [Xla.Tg(rho:Eco.nfsA)] (stage 51) under bright field illumination (a), blue light (b) or both (c, the tungsten lamp was dimmed compared to a). The bright green fluorescence from the transgene is seen in the eye. d: Dorsal view of a transgenic tadpole (stage 48) under bright field illumination. The position of the eyes (e), optic nerve (on), olfactory organ (o), cerebral hemispheres (c) and pineal (p) are indicated. A higher magnification image of the pineal under bright field (e) and blue light (f). GFP‐specific fluorescence is seen as a punctate pattern in pineal. g: The anterior portion of a transgenic juvenile frog illuminated with both tungsten lamp and blue light.
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Figure 4 . Confocal images of a retina from a transgenic tadpole [Xla.Tg(rho:Eco.nfsA)Zuber] produced using pXOP(−5500/+41)GFP. Two separate sections are shown (A and B, C and D). The images under bright field (A, C) were obtained with both bright field and blue light, so the GFP fluorescence (green) is visible in these images. Sections were also viewed for fluorescence from GFP (green) and Cy3 which revealed B630N rod opsin staining (blue) (B, D). Cones are indicated, as well as opsin‐ and GFP‐positive rods.
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| Stock Center |
RRID |
Generation |
DOB |
# Males |
# Females |
Availability |
Mutant Details |
| EXRC (Europe) |
EXRC_0049 |
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| NXR (US) |
NXR_0067 |
F2 |
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